SiRNA silencing of genes expressed in cancer
View Patent ↗The present invention provides nucleic acid-lipid particles comprising siRNA molecules that silence genes expressed in cancer (e.g., Eg5, EGFR or XIAP) and methods of using such nucleic acid-lipid particles to silence Eg5, EGFR or XIAP gene expression.
1. An siRNA molecule that silences Eg5 expression comprising a sense strand and a complementary antisense strand, wherein the antisense strand consists of the nucleic acid sequence of SEQ ID NO:44.
2. The siRNA molecule of claim 1 , wherein the siRNA molecule is chemically synthesized.
3. The siRNA molecule of claim 1 , wherein the sense strand comprises the nucleic acid sequence of SEQ ID NO:20.
4. The siRNA molecule of claim 1 , wherein the sense strand consists of the nucleic acid sequence of SEQ ID NO:43.
5. The siRNA molecule of claim 1 , wherein the siRNA molecule comprises at least one modified nucleotide.
6. The siRNA molecule of claim 5 , wherein the modified nucleotide is a 2′-O-methyl (2′OMe) nucleotide.
7. A composition comprising the siRNA molecule of claim 1 and a pharmaceutically acceptable carrier.
8. A nucleic acid-lipid particle comprising:
(a) the siRNA molecule of claim 1 ;
(b) a cationic lipid; and
(c) a non-cationic lipid.