Method for determining optical properties of turbid media
View Patent ↗There is provided a method for improving contrast and resolution of an optical image of an object obtained by time-resolved techniques such as Time Domain (TD) and Frequency Domain (FD). The method comprises obtaining a Temporal Point Spread Function (TPSF), and determining optical properties of volumes of interest (VOI), each volume being defined by an ensemble of equiprobable effective photon paths corresponding to a time point or time gate of the TPSF.
1. A method for determining an optical absorption coefficient of a volume of interest (VOI) in a turbid medium using time resolved optical modalities, said method comprising:
injecting light in said medium at an injection point;
detecting light exiting said medium at a detection point;
generating a Temporal Point Spread Function (TPSF) using said detected light;
correlating a predetermined time point or time gate of said TPSF with said VOI wherein said VOI comprises equiprobable effective photon paths; and
determining said optical absorption coefficient within said VOI using one or more characteristics of said TPSF.
2. The method as claimed in claim 1 wherein said absorption coefficient is determined as a function of a light transmission equation incorporating said one or more characteristics of said TPSF.
3. The method as claimed in claim 2 wherein said light transmission equation is the Beer-Lambert equation.
4. The method as claimed in claim 3 wherein said step of determining said absorption coefficient comprises the step of estimating an intensity value for said injected light to calculate said absorption coefficient using said Beer-Lambert equation.
5. The method as claimed in claim 4 further comprising the step of determining a concentration of a chromophore using said intensity value and said Beer-Lambert equation.
6. The method as claimed in claim 5 wherein the concentration of two or more chromophores is determined by obtaining two or more TPSF's at two or more wavelengths.
7. The method as claimed in claim 3 wherein said Beer-Lambert equation is used for determining concentration of a chromophore by using the ratio of intensity of said TPSF at said time point or time gate for said VOI with and without said chromophore.
8. The method as claimed in claim 4 wherein said intensity value is determined as a function of the rate of decay of the asymptotic portion of said TPSF and a time point or time gate within said asymptotic portion.
9. The method as claimed in claim 1 wherein said determination of said absorption coefficient is a relative determination of two VOI's.
10. The method as claimed in claim in claim 1 wherein said TPSF is obtained in transmission mode.
11. The method as claimed in claim 1 wherein said TPSF is obtained in the reflection mode.
12. The method as claimed in claim 1 wherein said VOI is an ellipsoid.
13. The method as claimed in claim 1 wherein said time point or time gate is equal to or greater than time point corresponding to the maximum of said TPSF.
14. The method as claimed in claim 1 wherein said optical absorption coefficient is mapped for more than one VOI thereby producing an image of optical absorption coefficient of said medium in a region of interest (ROI).
15. The method as claimed in claim 14 wherein a plurality of TPSF's are obtained from a plurality of injection/detection points configurations to produce a plurality of images of said VOI and wherein said images are combined to produce an image of said VOI with enhanced resolution.