Generation of plants with altered oil content
View Patent ↗The present disclosure is directed to plants and plant cells that display an altered oil content phenotype due to altered expression of a HIO nucleic acid. The disclosure is further directed to methods of generating plants with an altered oil content phenotype.
1. A method of producing a high oil phenotype in a plant, said method comprising:
a) introducing into progenitor cells of the plant a plant transformation vector comprising a heterologous constitutive promoter operatively linked to a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2 to produce transformed progenitor cells overexpressing the polypeptide;
b) growing the transformed progenitor cells to produce a transgenic plant overexpressing said polypeptide; and
c) identifying a high oil phenotype in said transgenic plant relative to a plant of the same species that does not comprise the plant transformation vector, thereby producing the high oil phenotype in said transgenic plant.
2. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
3. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
4. The method of claim 1 , wherein the nucleotide sequence encodes a polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.
5. A method of producing oil comprising:
growing a transgenic plant comprising a plant transformation vector comprising a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2, whereby overexpression of the polypeptide in the transgenic plant results in a high oil phenotype relative to a plant of the same species that does not comprise the plant transformation vector; and recovering the oil from said transgenic plant.
6. The method of claim 5 , wherein the oil is recovered from a seed of the plant, wherein said seed comprises the plant transformation vector.
7. The method of claim 5 , wherein the nucleotide sequence encodes a polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 2.
8. The method of claim 5 , wherein the nucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth as SEQ ID NO: 2.
9. The method of claim 5 , wherein the nucleotide sequence encodes a polypeptide consisting of the amino acid sequence set forth as SEQ ID NO: 2.