Biomarker panel for colorectal cancer
A panel of biomarkers has been identified for analysis of colorectal cancer. The panel, originally identified using a mouse colon cancer model, has been used to assess changes in human tissue from surgical and biopsy samples against a normal human control panel of biomarkers. The panel may be used for providing a cost effective, rapid, noninvasive procedure for risk assessment, early diagnosis, establishing prognosis, monitoring patient treatment, detecting relapse, and for the discovery of therapeutic intervention of colorectal cancer.
1 - 95 . (canceled)
96 . A method of detecting colorectal cancer in a subject, comprising:
(i) swabbing the rectum to collect colorectal cells from the subject;
(ii) measuring the expression level of IL-8 in the collected cells; and
(iii) comparing the level of IL-8 expression in the collected cells to the level of IL-8 expression in cells from normal rectal cells;
where an increase in expression of IL-8, relative to cells from normal rectal cells, is indicative of colorectal cancer.
97 . The method of claim 96 , wherein the expression level comprises measuring mRNA.
98 . The method of claim 96 , wherein the expression level comprises measuring cDNA.
99 . The method of claim 96 , wherein the measuring is via reverse transcriptase PCR.
100 . The method of claim 96 , wherein IL-8 comprises SEQ ID NO:1 or variants thereof.
101 . The method of claim 96 , wherein the measuring of IL-8 comprises PCR amplification with an oligonucleotide primer pair comprising SEQ ID NO:45 and 46.
102 . The method of claim 96 , further comprising measuring the expression level of at least one other biomarker selected from a COX-2, a CXCR2 and variants thereof.
103 . The method of claim 102 , wherein the COX-2 comprises SEQ ID NO:2 and variants thereof, and the CXCR2 comprises SEQ ID NO:3 and variants thereof.
104 . The method of claim 102 , wherein the expression level comprises measuring mRNA.
105 . The method of claim 104 , wherein the expression level comprises measuring cDNA.
106 . The method of claim 102 , wherein the measuring is via reverse transcriptase PCR.
107 . The method of claim 106 , wherein the measuring of IL-8 comprises PCR amplification with an oligonucleotide primer pair comprising SEQ ID NO:45 and 46; wherein the measuring of COX-2 comprises PCR amplification with an oligonucleotide primer pair comprising SEQ ID NO:47 and 48; and wherein the measuring of CXCR2 comprises PCR amplification with an oligonucleotide primer pair comprising SEQ ID NO:49 and 50.