METHOD FOR ANALYZING A FLUID SAMPLE
The invention relates to a method for analyzing a fluid sample, such as a body fluid sample in which, after loading a test element with a sample of a fluid, the fluid sample passes into a detection area of the test element and spreads out in the detection area, and in which a filling extent which characterizes the spread of the fluid sample in the detection area is determined using a photometric measurement of the fluid sample in the detection area, wherein the photometric measurement is carried out by means of a first light which is irradiated onto the fluid sample in the detection area, said first light having a first wavelength for which, when at least one chemical parameter of the fluid is varied, the fluid has an essentially constant optical behaviour with respect to at least one spectroscopic parameter selected from a group of spectroscopic measurement parameters consisting of absorption, transmission, remission and fluorescence, and wherein the at least one chemical parameter of the fluid comprises at least one of the following properties: chemical composition, concentration and activity of one or more compounds contained in the fluid.
1 . A method for analyzing a fluid sample, comprising:
introducing the fluid sample to a test element, the test element comprising at least at least a measurement area and a detection area, the sample sequentially or essentially simultaneously spreading into the detection area and the measurement area;
photometrically measuring an extent of filling of the detection area by the fluid sample, said measuring comprising:
irradiating the sample in the detection area with a first light and measuring at least one spectroscopic parameter selected from the group of parameters consisting of absorption, transmission, remission and fluorescence, the first light having a first wavelength at which the at least one spectroscopic parameter measured therefrom is substantially independent of at least one chemical parameter of the sample comprising at least one of composition, concentration of compounds in the sample and activity of such compounds; and
correlating the measured spectroscopic parameter to the extent of the filling of the detection area; and
analyzing the sample in the measurement area.
2 . The method according to claim 1 , wherein the detection area is wetted by the fluid sample when the fluid sample spreads out in the detection area.
3 . The method according to claim 1 , wherein the detection area is moistened by the fluid sample when the fluid sample spreads out by the detection area.
4 . The method according to claim 1 , wherein the measuring of the extent of filling is carried out in a time-resolved manner.
5 . The method according to claim 4 , wherein a temporal curve of the at least one spectroscopic parameter is measured.
6 . The method according to claim 5 , wherein the temporal curve is correlated to a value of the extent of filling.
7 . The method according to any one of claim 4 , wherein the extent of filling is determined from measured values for the at least one spectroscopic parameter which are measured at a pre-determined time after the start of the irradiating of the fluid sample with the first light.
8 . The method according to claim 7 , wherein the pre-determined time is selected to be a time at which at least one spectroscopic parameter comprises an extreme value.
9 . The method according to claim 1 , characterized in that the test element comprises one of a test strip, a test field, a test element having a cavity structure for holding the fluid sample, and a micro-fluidic test element.
10 . The method according to claim 1 , wherein the analyzing the sample comprises carrying out an analysis measurement.
11 . The method according to claim 10 , wherein the analysis measurement comprises one of an electrochemical measurement and a photometric measurement.
12 . The method according to claim 10 , wherein the analysis measurement is based on a function of a value measured for the extent of filling, said function comprising a data signal which is derived from electronically accessible information about the measured value.
13 . The method according to claim 10 , wherein the analysis measurement at least partially overlaps temporally with the photometric measurement.
14 . The method according to claim 13 , wherein the analysis measurement begins before or after the photometric measurement.
15 . The method according to claim 13 , wherein the analysis measurement ends before or after the photometric measurement.
16 . The method according to claim 10 , wherein the analysis measurement is carried out in the measurement area of the test element.
17 . The method according to claim 1 , wherein the measurement area and the detection area at least partially overlap.
18 . The method according to claim 17 , wherein the measurement area and the detection area are the same.
19 . The method for analyzing a fluid sample, comprising:
introducing the sample to a test element, wherein the fluid sample passes into a detection area of the test element and spreads out in the detection area; and
determining a filling extent, said filling extent characterizing the spread of the fluid sample in the detection area, said determining comprising a photometric measurement of the fluid sample in the detection area, wherein the photometric measurement comprises irradiating a first light onto the fluid sample in the detection area, said first light having a first wavelength for which the fluid has an essentially constant optical behaviour with respect to at least one spectroscopic parameter when at least one chemical parameter of the fluid is varied, said spectroscopic parameter being selected from a group of spectroscopic parameters consisting of absorption, transmission, remission and fluorescence, wherein the at least one chemical parameter of the fluid characterizes at least one of composition, concentration and activity of one or more compounds contained in the fluid.