Chimeric and humanized antibodies to α5β1 integrin that modulate angiogenesis
View Patent ↗The present invention provides chimeric and humanized antibodies that specifically recognize α5β1 integrin, and methods for using the antibodies for reducing or inhibiting angiogenesis in a tissue. Also provided are methods of determining therapeutically acceptable doses of the antibodies and pharmaceutical compositions including the same.
1. An isolated nucleic acid encoding a polypeptide comprising a heavy chain amino acid sequence selected from the group consisting of SEQ ID NOs: 2-6, 16, 20, 25, 28, and 31; and a light chain amino acid sequence selected from the group consisting of SEQ ID NOs: 8-12, 18, 22, 26, and 32.
2. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:2 and 8.
3. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:3 and 9.
4. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:4 and 10.
5. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:5 and 11.
6. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:6 and 12.
7. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:16 and 18.
8. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:20 and 22.
9. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:25 and 26.
10. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:28 and 26.
11. The isolated nucleic acid of claim 1 , wherein the polypeptide comprises the amino acid sequences of SEQ ID NOs:31 and 32.