Method to repair a damaged intervertebral disc through the use of a bioadhesive, thermogelling hydrogel
View Patent ↗The present invention is directed to a bioadhesive thermogelling hydrogel composition for repairing and/or augmenting an intervertebral disc. The bioadhesive thermogelling hydrogel composition can be cross linked with the surrounding tissue so as to potentially serve as a nucleus pulposus replacement or augmentation along with serving to repair annular tears or fissure. The bioadhesive thermogelling hydrogel composition may include three main components: a thermal responsive polymer, an amine-containing polymer and a crosslinking component. All three components can be modified and combined in numerous ways to serve the need of the system as long as the amine-containing component is kept separate from the crosslinking component until the components are injected. The incorporation of a two-part crosslinking thermal responsive hydrogel permits smaller amounts of the crosslinking component to be used and enables the crosslinking dialdehyde to be delivered locally into the tissue that will react with the hydrogel. This helps prevent damage to tissues away from the hydrogel.
1. A bioadhesive thermogelling hydrogel composition for repairing or augmenting an intervertebral disc and comprising: a thermoresponsive polymer component which is poly(n-isopropyl acrylamide); an amine-containing polymer component which is poly(ethylene imine) that is further reacted with a carbonate group by the creation of stable urethane linkages; and a crosslinker which is a dialdehyde component that is selected from the group consisting of butanedial, glutaraldehyde, and mixtures thereof, and wherein the dialdehyde component is separated from the poly(ethylene imine) until the poly(n-isopropyl acrylamide, the dialdehyde component, and the poly(ethylene imine) are injected into an intervertebral disc such that the dialdehyde component crosslinks with the poly(ethylene imine) and the composition solidifies in situ, and wherein the solidified in situ composition is capable of crosslinking to tissue surrounding the composition.
2. The composition of claim 1 , wherein the poly(n-isopropyl acrylamide) is copolymerized with poly(ethylene glycol) prior to be combined with the poly(ethylene imine) and glutaraldehyde.
3. The composition of claim 2 , wherein the composition has an adhesion strength between about 0.20 MPa to about 0.34 MPa.
4. The composition of claim 2 , wherein the poly(ethylene glycol) copolymerized poly(n-isopropyl acrylamide) comprises about 15% by weight of the composition.
5. The composition of claim 4 , wherein the poly(ethylene imine) comprises about 8.5% by weight of the composition.
6. The composition of claim 1 , wherein the poly (n-isopropyl acrylamide) and the poly (ethylene imine) are injected first before injection of the dialdehyde component.
7. The composition of claim 1 , wherein the poly(n-isopropyl acrylamide) and the poly(ethylene imine) are simultaneously injected into the intervertebral disc.
8. A bioadhesive thermogelling hydrogel composition for an intervertebral disc and comprising: a thermoresponsive polymer component which comprises poly(n-isopropyl acrylamide) (PNIPPAm); an amine-containing polymer component which comprises poly(ethylene) imine that is further reacted with a carbonate group by the creation of stable urethane linkages; and a crosslinker which comprises a di-functional dialdehyde component that is selected from the group consisting of butanedial, glutaraldehyde, and mixtures thereof, wherein the components are injectable into an intervertebral disc where the composition solidifies in situ, wherein the aldehyde component is separated from the poly(ethylene imine) until they are injected into the intervertebral disc such that the dialdehyde component crosslinks with the poly(ethylene imine) and wherein the solidified in situ composition is capable of crosslinking to tissue surrounding the composition.
9. The composition of claim 8 , wherein the thermoresponsive polymer component and the amine-containing polymer component are blended in an aqueous solution.
10. The composition of claim 8 , wherein the thermoresponsive component and the amine-containing component exist as copolymers.
11. The composition of claim 8 , wherein the thermoresponsive polymer component is reacted to include aldehyde groups.
12. The composition of claim 8 , wherein the thermoresponsive polymer component and the amine-containing component are inserted into the intervertebral disc collectively and wherein the crosslinker is inserted into the intervertebral disc after the thermoresponsive polymer component and the amine-containing component have been inserted into the intervertebral disc.
13. The composition of claim 8 , wherein the thermoresponsive polymer component and the crosslinker are inserted into the intervertebral disc collectively and wherein the amine-containing component is inserted into the intervertebral disc after the thermoresponsive polymer component and the crosslinker have been inserted into the intervertebral disc.
14. The composition of claim 8 , wherein the thermoresponsive polymer component comprises homopolymers of poly-(n-ispropylacrylamide) (PNIPAAm), copolymers of poly-(n-isopropylacrylamide) (PNIPAAm) with polyethylene glycol (PEG), and mixtures thereof.
15. The composition of claim 14 , wherein the thermoresponsive polymer component comprises copolymers of PNIPAAm-PEG.
16. The composition of claim 8 , wherein the crosslinker is glutaraldehyde.
17. The composition of claim 8 wherein the thermoresponsive polymer component is grafted with a whole or parts of the amine-containing polymer.