Predictive autofocusing
View Patent ↗A method of autofocusing includes capturing first, second and third images of a sample, at respective first, second and third sample distances and respective first, second and third lateral positions determined with respect to an objective; determining a quantitative characteristic for the first, second and third images; determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third images; and capturing a primary image of the sample at the primary sample distance and at a primary lateral position that is offset from the first, second and third lateral positions.
1. A method of autofocusing a sample to obtain a plurality of sequential primary images representative of the sample, the method comprising:
capturing a first auxiliary image of the sample, the first auxiliary image captured at a first sample distance and at a first lateral position determined with respect to an objective;
determining a quantitative characteristic for the first auxiliary image;
capturing a second auxiliary image of the sample, the second auxiliary image captured at a second sample distance and at a second lateral position that is offset from the first lateral position;
determining a quantitative characteristic for a second auxiliary image;
capturing a third auxiliary image of the sample, the third auxiliary image captured at a third sample distance and at a third lateral position that is offset from the first and second lateral positions;
determining a primary sample distance based upon at least the quantitative characteristics for the first, second, and third auxiliary images; and
capturing a primary image of the sample at the primary sample distance determined from the quantitative characteristics for the first, second and third auxiliary images and at a primary lateral position that is offset from the first, second and third lateral positions.
2. The method of claim 1 , wherein the sample distances vary in a vertical direction between the objective lens and the sample.
3. The method of claim 2 , wherein the lateral positions vary substantially orthogonally with respect to the vertical direction.
4. The method of claim 2 , wherein the sample is moved continuously between capturing of the first auxiliary image and the primary image in the lateral direction relative to the objective.
5. The method of claim 1 , wherein determining a quantitative characteristic comprises determining a difference in intensities between each pixel and at least one other pixel in a captured image.
6. The method of claim 1 , wherein determining a quantitative characteristic comprises determining, for each pixel in a captured image, a difference in intensities between each respective pixel and a neighboring pixel laterally separated from the respective pixel by at least one other pixel.
7. The method of claim 1 , wherein determining a primary sample distance comprises interpolating the primary sample distance in accordance with a quadratic or Lorentzian distribution based upon the quantitative characteristics for the first, second and third auxiliary images.
8. The method of claim 1 , wherein determining a quantitative characteristic comprises determining, for each pixel in a captured image, a difference in intensities between each respective pixel and a neighboring pixel laterally separated from the pixel by at least one other pixel, and summing the squared difference in intensities for each pixel across the height and width of the image.
9. The method of claim 8 , wherein determining a primary sample distance comprises interpolating the primary sample distance by fitting at least the quantitative characteristics or the first, second and third images to a Lorentzian curve and identifying a maxima on the curve.
10. The method of claim 9 , wherein one primary image of the sample is used in determining the primary sample distance of the sequentially next primary image of the sample.
11. The method of claim 8 , wherein determining a primary sample distance comprises interpolating the primary sample distance by fitting at least an inverse of the quantitative characteristic for the first image and an inverse of the quantitative characteristic for the second image to a quadratic curve and identifying a minima on the curve.
12. The method of claim 11 , wherein one primary image of the sample is used in determining the primary sample distance of the sequentially next primary image of the sample.
13. An imaging device comprising:
an image sensor that generates a plurality of sequential primary images of a sample;
an objective lens;
a stage that supports the sample to be imaged; and
a controller that adjusts a sample distance between the objective lens and the sample along an optical axis to autofocus the images as the relative positions of the sample and the objective lens are varied in a lateral direction substantially orthogonal to the optical axis, wherein the controller
captures a first auxiliary image of the sample, the first auxiliary image captured at a first sample distance and at a first lateral position,
determines a quantitative characteristic for the first auxiliary image,
captures a second auxiliary image of the sample, the second auxiliary image captured at a second sample distance and at a second lateral position that is offset from the first lateral position,
determines a quantitative characteristic for the second auxiliary image,
captures a third auxiliary image of the sample, the third auxiliary image captured at a third sample distance and at a third lateral position that is offset from the first and second lateral positions,
determines a quantitative characteristic for the third auxiliary image,
determines a primary sample distance based upon at least the quantitative characteristics for the first, second and third auxiliary images, and
captures a primary image of the sample at the primary sample distance determined from the quantitative characteristics for the first, second and third auxiliary images and at a primary lateral position that is offset from the first, second and third lateral positions.
14. The imaging device of claim 13 , wherein one primary image of the sample is used in determining the primary sample distance of the sequentially next primary image of the sample.
15. The imaging device of claim 13 , wherein the imaging device comprises a digital optical microscope.
16. The imaging device of claim 13 , wherein the sample distances vary in a vertical direction between the objective lens and the sample.
17. The imaging device of claim 16 , wherein the lateral positions vary substantially orthogonally with respect to the vertical direction.
18. The imaging device of claim 17 , wherein the sample is moved continuously between capturing of the first auxiliary image and the primary image in the lateral direction relative to the objective.
19. The imaging device of claim 13 , wherein determining a quantitative characteristic comprises determining, for each pixel in a captured image, a difference in intensities between the pixel and a neighboring pixel laterally separated from the pixel by at least one other pixel, and summing the squared difference in intensities for each pixel across the height and width of the image.
20. The imaging device of claim 19 , wherein determining a primary sample distance comprises interpolating the primary sample distance by fitting at least the quantitative characteristics for the first, second and third auxiliary images to a Lorentzian curve and identifying a maxima on the curve.
21. The imaging device of claim 19 , wherein determining a primary sample distance comprises interpolating the primary sample distance by fitting at least an inverse of the respective quantitative characteristics for the first, second and third images to a quadratic curve and identifying a minima on the curve.