Use of glycosaminoglycans to reduce non-specific binding in immunoassays
View Patent ↗An immunoassay reagent is provided which comprises an analyte binding agent in a diluent, and a glycosaminoglycan in an amount sufficient to reduce non-specific binding in an assay of a sample for the analyte. Provided is such an immunoassay reagent in which the analyte is troponin I, the analyte binding agent is a biotinylated anti-troponin I antibody, and the glycosaminoglycan is chondroitin sulfate. A sample composition is also provided which comprises a sample to be assayed for the presence of an analyte, an analyte binding agent, and a glycosaminoglycan other than heparin. Further provided is a method of detecting an analyte in a sample, in which non-specific binding is reduced in the method using a glycosaminoglycan.
1. An immunoassay reagent comprising: a troponin I binding agent in a diluent; and chondroitin sulfate in an amount sufficient to reduce non-specific binding in an assay of a sample for the troponin I, wherein the chondroitin sulfate is present in an amount from about 0.25 mg/mL to about 4 mg/mL.
2. The immunoassay reagent of claim 1 wherein the chondroitin sulfate is present in an amount of about 1 mg/mL.
3. The immunoassay reagent of claim 1 wherein the troponin I binding agent is an antibody.
4. A sample composition comprising:
a sample to be assayed for the presence of troponin I; and
the immunoassay reagent of claim 1 .
5. The sample composition of claim 4 wherein the sample is a serum sample.
6. The sample composition of claim 4 wherein the sample is a plasma sample containing ethylenediamine tetraacetic acid.
7. The sample composition of claim 4 wherein the troponin I binding agent is an antibody.
8. A method of detecting troponin I in a sample, the method comprising:
combining a sample to be assayed for the presence of troponin I with the immunoassay reagent of claim 1 , so as to form a complex of any troponin I present in the sample and the troponin I binding agent, wherein the chondroitin sulfate reduces non-specific binding in the method; and
detecting the resulting complex so as to detect the troponin I.