The present invention provides methods, kits, and compositions related to human papillomavirus (HPV) primers, which may be used, for example, in nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays.
1. A composition configured to hybridize to target nucleic acids from a plurality of different HPV types, said composition comprising an isolated nucleic acid sequence, wherein the nucleotide sequence of said isolated nucleic acid sequence consists of SEQ ID NO:142 or SEQ ID NO:143.
2. The composition of claim 1 , further comprising a second isolated nucleic acid sequence different from said isolated nucleic acid sequence, wherein the nucleotide sequence of said second isolated nucleic acid sequence consists of SEQ ID NO:142 or SEQ ID NO:143.
3. The composition of claim 1 , further comprising at least 20 additional isolated nucleic acid sequences, wherein the nucleotide sequences of said additional isolated nucleic acid sequences each consist of a nucleotide sequence shown in SEQ ID NOs:1-123 and 142-154.
4. A kit comprising a composition configured to hybridize to target nucleic acids from a plurality of different HPV types, said composition comprising an isolated nucleic acid sequence, wherein the nucleotide sequence of said isolated nucleic acid sequence consists of SEQ ID NO:142 or SEQ ID NO:143.
5. The kit of claim 4 , wherein said composition further comprises a second isolated nucleic acid sequence different from said isolated nucleic acid sequence, wherein the nucleotide sequence of said second isolated nucleic acid sequence consists of SEQ ID NO:142 or SEQ ID NO:143.
6. The kit of claim 4 , further comprising at least 20 additional isolated nucleic acid sequences, wherein the nucleotide sequences of said additional isolated nucleic acid sequences each consist of a nucleotide sequence shown in SEQ ID NOs:1-123 and 142-154.
7. A method for detecting at least one HPV sequence in a sample, comprising using a composition configured to hybridize to target nucleic acids from a plurality of different HPV types, said composition comprising an isolated nucleic acid sequence, wherein the nucleotide sequence of said isolated nucleic acid sequence consist of SEQ ID NO:142 or SEQ ID NO:143.
8. The method of claim 7 , wherein said detecting allows typing of said HPVsequence.
9. The method of claim 7 , wherein said using comprises sequencing with said nucleic acid sequence.
10. The method of claim 7 , wherein said using comprises amplifying said HPV sequence with said nucleic acid sequence.