Method for manufacture of polyphenols by using seeds as starting material
View Patent ↗To provide a method for the manufacture of polyphenols that enables industrial manufacture of polyphenols containing water-soluble proanthocyanidins from grape seeds. The seeds of grape berries dried after squeezing out the juice are separated into grape seeds and peel with a separation apparatus, and the grape seeds are stirred in a washing machine to remove contamination thoroughly. The grape seeds are charged into a boiling kettle and heated for the prescribed time at the prescribed temperature. Then, germination is induced in a curing kettle maintained at the prescribed temperature preferred for the germination. The grape seeds in which germination or germination and differentiation have advanced are crushed, mixed with water to obtain an emulsified state, and a salt is added, if desired. After a known desalting treatment has been conducted with respect to an aqueous solution portion, water is evaporated with a vacuum apparatus to obtain polyphenols comprising proanthocyanidins converted to a water soluble form.
1. A method of preparing grape seeds for extraction, comprising:
removing juice from the seeds;
drying the grape seeds after removing the juice step;
heating the grape seeds after the drying step so as not to sterilize the grape seeds; wherein the heating is carried out using a temperature range of 50-70° C.;
hydrating the grape seeds with water;
inducing germinating of the grape seeds, after the hydrating step by maintaining the grape seeds at a temperature range optimum for germination, until germination of the grape seeds occurs; and,
extracting water soluble polyphenols from the geminated grape seeds after the germinating step, wherein the germinated grape seeds are crushed, mixed with water to create an emulsion and the water is evaporated from the emulsion to form a concentrate of water soluble polyphenols.
2. The method of preparing grape seeds for extraction, as recited in claim 1 , wherein after the drying step, the grape seeds have a water content of 9-12%.
3. The method of preparing grape seeds for extraction, as recited in claim 1 , wherein the hydrating step is after the heating step and after the hydrating step the grape seeds have a water content of at least 17 percent.
4. A method for extracting water soluble polyphenols from dried grape seeds comprising:
inducing germination of the grape seeds by drying the grape seeds, then heating and hydrating the grape seeds to awaken the grape seeds from a dormant state and to increase the water content of the seed to at least 17 percent, wherein once the grape seeds are awakened, the grape seeds are maintained at a temperature range optimum for germination, which is 20 to 45 degrees centigrade or 50 to 70 degrees centigrade, until the grape seeds germinate; and,
extracting the water soluble polyphenols from the germinated grape seeds, including crushing the germinated grape seeds, mixing the crushed grape seeds with water to create an emulsion and evaporating water from the emulsion.
5. The method extracting water soluble polyphenols from dried grape seeds, as recited in claim 4 , wherein during the inducing germination step, when the grape seeds are dried, the grape seeds have a water content of 9-12%.
6. The method extracting water soluble polyphenols from dried grape seeds, as recited in claim 4 , wherein during the inducing germination step, when the grape seeds are hydrated, the grape seeds have a water content of at least 17 percent.