COT102 insecticidal cotton
View Patent ↗The present application relates to an insect resistant transgenic cotton plant. In particular, it relates to a specific event, designated COT102. The application also relates to polynucleotides which are characteristic of the COT102 event, plants comprising said polynucleotides, and methods of detecting the COT102 event. The COT 102 event exhibits a novel genotype comprising two expression cassettes. The first cassette comprises a suitable promoter for expression in plants operably linked to a gene that encodes a VIP3A insecticidal toxin, useful in controlling a vide spectrum of lepidopteran insect pests, and a suitable polyadenylation signal. The second cassette comprises a gene which, when expressed, can be used as a selectable marker.
1. A method of detecting plant material from a COT102 cotton event plant comprising:
(a) obtaining a sample for analysis;
(b) providing DNA from the sample;
(c) providing a pair of primers comprising a first primer and a second primer which function together in the presence of a cotton event COT102 DNA template in a sample to produce an amplicon diagnostic for the cotton event COT102;
(d) carrying out a PCR amplification reaction which results in an amplicon; and
(e) detecting the presence of the resulting amplicon; wherein the amplicon comprises SEQ ID NO: 1 or SEQ ID NO: 2.
2. The method according to claim 1 , wherein the first primer is designed to bind to a COT102 insertion sequence and the second primer is designed to bind to a flanking genomic DNA sequence downstream of the 3′ end of the COT102 insertion site or to a flanking genomic DNA sequence upstream of the 5′ end of the COT102 insertion site.
3. The method according to claim 2 , wherein the first primer has the sequence of SEQ ID NO: 3 and the second primer has the sequence of SEQ ID NO: 4.
4. The method according to claim 2 , wherein the first primer has the sequence of SEQ ID NO: 19 and the second primer has the sequence of SEQ ID NO: 18.
5. A kit for detecting the presence of cotton event COT102 nucleic acids in a biological sample, the kit comprising a first primer and a second primer, wherein the first primer and the second primer, when used together in a PCR reaction, produce an amplicon that comprises SEQ ID NO: 1 or SEQ ID NO: 2.
6. The kit according to claim 5 , wherein the first primer is designed to bind to a COT102 insertion sequence and the second primer is designed to bind to a flanking genomic DNA sequence downstream of the 3′ end of the COT102 insertion site or to a flanking genomic DNA sequence upstream of the 5′ end of the COT102 insertion site.
7. The kit according to claim 6 , wherein the first primer has the sequence of SEQ ID NO: 3 and the second primer has the sequence of SEQ ID NO: 4.
8. The kit according to claim 6 , wherein the first primer has the sequence of SEQ ID NO: 19 and the second primer has the sequence of SEQ ID NO: 18.