Chelating agents with lipophilic carriers
View Patent ↗Compounds useful for associating with nanoparticle or microparticle emulsions to obtain magnetic resonance images permit control of the relaxivity of the signal and readily associate with the particulate components.
1. A compound of the formula:
wherein Ch represents a chelating moiety selected from the group consisting of porphyrins, ethylenediaminetetraacetic acid (EDTA), diethylenetriamine-N,N,N′,N″,N″-pentaacetate (DTPA), 1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7 (ODDA), 16-diacetate,N-2-(azol-1(2)-yl)ethyliminodiacetic acids, 1,4,7,10-tetraazacyclododecane-N,N′,N″,N′″-tetraacetic acid (DOTA), 1,7,13-triaza-4,10,16-trioxacyclo-octadecane-N,N′,N′″-triacetate (TTTA), tetraethylene glycols, 1,5,9-triazacyclododecane-N,N′,N″-tris(methylenephosphonic acid (DOTRP), and N,N′,N″-trimethylammonium chloride (DOTMA);
m is 0-3;
each R 1 is independently a non-interfering substituent selected from the group consisting of halo, OR, NR 2 , SR, CN, NO 2 , SO 3 H, and R where R is alkyl or alkenyl optionally substituted by halo, or ═O, and optionally containing a heteroatom, such as O, S or N;
1 is 0-2;
Z is S or O;
R 2 is H or alkyl (1-4C);
n is 1; and
each R 3 is independently an optionally substituted saturated or unsaturated hydrocarbyl group containing at least 10 C; and
spacer is —CH 2 CH 2 — and/or includes a peptide, a pseudopeptide, or a polyalkylene glycol, optionally containing a cleavage site.
2. The compound of claim 1 , wherein the spacer is CH 2 CH 2 and R 2 is H.
3. The compound of claim 1 , wherein R 2 is H, 1 is 0 or 1 and m is 1 or 0.
4. The compound of claim 1 , wherein each R 3 COO is a residue of a naturally occurring fatty acid or a mixture of said residues.
5. The compound of claim 1 , wherein R 1 is CH 3 O.
6. The compound of claim 1 , wherein the spacer is a peptide or a polyalkylene glycol.
7. The compound of claim 1 , which further comprises, associated with Ch, a paramagnetic metal.
8. A composition which comprises the compound of claim 7 associated with lipophilic nanoparticles or microparticles, and wherein said particles contain at least 2,000 copies of said compound.
9. The composition of claim 8 , wherein the nanoparticles or microparticles further contain a targeting agent.
10. The composition of claim 8 , wherein said microparticles or nanoparticles further comprise a biologically active agent.
11. The composition of claim 8 , wherein said microparticles or nanoparticles are liposomes, oil droplets, perfluorocarbon nanoparticles, lipid-coated protein particles, or lipid-coated polysaccharides.
12. A method to obtain a magnetic resonance image which method comprises imaging a tissue which is associated with the composition of claim 9 .
13. The compound of claim 7 , wherein the paramagnetic metal ion is Gd(III), Mn(II), iron, europium and/or dysprosium.
14. The compound of claim 13 , wherein the paramagnetic metal ion is Gd(III).
15. A composition which comprises the compound of claim 13 associated with lipophilic nanoparticles or microparticles, and wherein said particles contain at least 2,000 copies of said compound.
16. The composition of claim 15 , wherein the nanoparticles or microparticles further contain a targeting agent.
17. A method to obtain a magnetic resonance image which method comprises imaging a tissue which is associated with the composition of claim 16 .
18. A composition which comprises the compound of claim 14 associated with lipophilic nanoparticles or microparticles, and wherein said particles contain at least 2,000 copies of said compound.
19. The composition of claim 18 , wherein the nanoparticles or microparticles further contain a targeting agent.
20. A method to obtain a magnetic resonance image which method comprises imaging a tissue which is associated with the composition of claim 19 .
21. A compound of the formula:
wherein Ch represents a chelating moiety;
m is 0-3;
each R 1 is independently selected from the group consisting of halo, OR, NR 2 , SR, CN, NO 2 , SO 3 H, and R where R is alkyl or alkenyl optionally substituted by halo, or ═O, and optionally containing a heteroatom, such as O, S or N;
1 is 0-2;
Z is S or O;
R 2 is H or alkyl (1-4C);
n is 1; and
each R 3 is independently an optionally substituted saturated or unsaturated hydrocarbyl group containing at least 10C; and
spacer is —CH 2 CH 2 — and/or includes a peptide, a pseudopeptide, or a polyalkylene glycol, optionally containing a cleavage site;
which further comprises, associated with CH, a paramagnetic metal ion.
22. A composition which comprises the compound of claim 21 associated with lipophilic nanoparticles or microparticles, and wherein said particles contain at least 2,000 copies of said compound.
23. The composition of claim 22 , wherein the nanoparticles or microparticles further contain a targeting agent.
24. The composition of claim 22 , wherein said microparticles or nanoparticles further comprise a biologically active agent.
25. The composition of claim 22 , wherein said microparticles or nanoparticles are liposomes, oil droplets, perfluorocarbon nanoparticles, lipid-coated protein particles, or lipid-coated polysaccharides.
26. A method to obtain a magnetic resonance image which method comprises imaging a tissue which is associated with the composition of claim 23 .
27. The compound of claim 21 , wherein the paramagnetic metal ion is Gd(III), Mn(II), iron, europium and/or dysprosium.
28. The compound of claim 27 , wherein the paramagnetic metal ion is Gd(III).