Nano-chem-FET based biosensors
View Patent ↗Methods of detecting a component of interest, a change in charge, a pH, a cellular response using nanosensors are provided. Nanosensors, including nanowires and nanowire arrays comprising functionalized and/or non-functionalized nanowires are provided. Nanosensors are used for detection in cellular fragmentation, multiple concentration analysis, glucose detection, and intracellular analysis.
1. A method of detecting glucose, the method comprising:
(a) providing one or more nanowires comprising a metal coating and glucose oxidase immobilized on the one or more nanowires or proximal thereto;
(b) contacting the nanowires with a test solution; wherein any glucose present in the test solution is oxidized by the glucose oxidase resulting in a change in pH of the test solution, wherein the change in pH produces a signal in the nanowires; and,
(c) detecting the signal, thereby detecting the glucose in the test solution.
2. The method of claim 1 , wherein the test solution comprises one or more cells, one or more cellular fragments, a tissue sample, a cell lysate, or a blood sample.
3. The method of claim 1 , wherein the one or more nanowires are positioned in one or more microwells or within a microfluidic device.
4. The method of claim 1 , wherein the one or more nanowires comprise a nanowire array.
5. The method of claim 1 , wherein the one or more nanowires comprise glucose oxidase immobilized thereon.
6. The method of claim 1 , wherein the one or more nanowires comprise silicon nanowires.
7. The method of claim 6 , wherein the one or more silicon nanowires are coated with a layer of metal.
8. The method of claim 7 , wherein the metal comprises zinc.