Colloidal metal conjugates
View Patent ↗Colloidal metal conjugates can be produced in high concentrations suitable for direct use, for example, in immunoassays. The colloidal metal conjugates can be used in devices for qualitative, semi-quantitative, or quantitative determination of the presence of compounds in samples, including biological samples.
1. A method of producing an assay device comprising:
providing a plurality of colloidal metal particles;
mixing the plurality of colloidal metal particles with an amount of an affinity reagent substantially equal to the amount that can be conjugated to the colloidal particles to form the conjugate; and
depositing the conjugate directly on a substrate, wherein depositing the conjugate directly on a substrate includes placing the conjugate on a surface of the substrate without purifying the conjugate.
2. The method of claim 1 , wherein depositing the conjugate directly on a substrate includes placing the conjugate on a surface of the substrate without concentrating the conjugate.
3. The method of claim 1 , wherein the plurality of colloidal metal particles has a maximum optical density of at least 2.
4. The method of claim 3 , wherein the plurality of colloidal metal particles has a maximum optical density of less than 8.
5. The method of claim 1 , wherein the plurality of colloidal metal particles has a maximum optical density of between 3 and 7.5.
6. The method of claim 1 , wherein the affinity reagent is a biomolecule.
7. The method of claim 1 , wherein the affinity reagent is an antibody.
8. The method of claim 1 , wherein the affinity reagent is a mixture of an antigen-specific antibody and a non-specific antibody.
9. The method of claim 1 , wherein the metal is gold, platinum, silver, iron, copper, selenium, chromium, vanadium, titanium, or manganese, or an alloy thereof.
10. The method of claim 1 , wherein the metal is gold.
11. A method of producing an assay device comprising:
providing a plurality of colloidal metal particles;
mixing the plurality of colloidal metal particles with an amount of an affinity reagent substantially equal to the amount that can be conjugated to the colloidal particles to form the conjugate; and
depositing the conjugate directly on a substrate, wherein the affinity reagent is a mixture of an antigen-specific antibody and a non-specific antibody.
12. The method of claim 11 , wherein depositing the conjugate directly on a substrate includes placing the conjugate on a surface of the substrate without concentrating the conjugate.
13. The method of claim 11 , wherein the plurality of colloidal metal particles has a maximum optical density of at least 2.
14. The method of claim 13 , wherein the plurality of colloidal metal particles has a maximum optical density of less than 8.
15. The method of claim 11 , wherein the plurality of colloidal metal particles has a maximum optical density of between 3 and 7.5.
16. The method of claim 11 , wherein the affinity reagent is a biomolecule.
17. The method of claim 11 , wherein the affinity reagent is an antibody.
18. The method of claim 11 , wherein the metal is gold, platinum, silver, iron, copper, selenium, chromium, vanadium, titanium, or manganese, or an alloy thereof.
19. The method of claim 11 , wherein the metal is gold.