Double-structured tissue implant and a method for preparation and use thereof
View Patent ↗A double-structured tissue implant and a method for preparation and use thereof for implantation into tissue defects. The double-structured tissue implant comprising a primary scaffold and a secondary scaffold consisting of a soluble collagen solution in combination with a non-ionic surfactant generated and positioned within the primary scaffold. A stand alone secondary scaffold implant or unit. A process for preparation of the double-structured implant or the stand alone secondary scaffold comprising lyophilization and dehydrothermal treatment.
1. A double-structured collagen implant comprising
an acellular, three-dimensional scaffold comprising a plurality of pores; and
a composition comprising a collagenous solution or gel and a surfactant that fills the plurality of pores to form a fibrous and cross-linked collagen network within said plurality of pores; and
a pharmaceutical agent incorporated into the scaffold, composition, or both.
2. The double-structured collagen implant of claim 1 , wherein the pharmaceutical agent is selected from growth and morphogenic factors, cytokines, membrane associated factors that promote growth and morphogenesis, cell attachment or adhesion proteins, hormones, pericellular matrix molecules, nutrients, nucleic acids, anti-neoplastic agents, anti-neoplastic agents, anti-inflammatory agents, enzymes, and metabolic inhibitors.
3. The double-structured collagen implant of claim 2 , wherein the anti-neoplastic agent is methotrexate or aminopterin.
4. The double-structured collagen implant of claim 2 , wherein the metabolic inhibitor is an RNAi, a cyclohexamide, or a steroid.
5. The double-structured collagen implant of claim 1 , further comprising living cells.
6. The double-structured collagen implant of claim 1 , wherein the implant comprises a primary scaffold comprising a porous collagen-containing material and a secondary scaffold comprising a soluble collagen and a nonionic surfactant.
7. The double-structured collagen implant of claim 6 , wherein the soluble collagen is soluble in acidic or basic solutions.
8. The double-structured collagen implant of claim 6 , wherein the nonionic surfactant comprises a copolymer of polyoxyethylene and polyoxypropylene.