Reagency and Method for Preventing Time-Dependent Expression in Biological Cells
The present invention relates to a reagent for prevention of time-dependent, induced expression in biological cells in a sample. It likewise relates to a method for this purpose and also to cell preparations treated in this manner, the reagent for prevention of time-dependence induced expression in biological cells in a sample ex vivo containing a formaldehyde donor.
1 . A method for prevention of time-dependent illegitimate expression of mRNA in a biological cell in a sample ex vivo comprising mixing the sample with a reagent comprising a formaldehyde donor.
2 . The method according to claim 1 , wherein the resulting mixture comprises the formaldehyde donor in a concentration of ≦0.075% (w/v).
3 . The method according to claim 2 , wherein the resulting mixture comprises the formaldehyde donor in a concentration of ≦0.045% (w/v), advantageously ≦0.025% (w/v).
4 . The according to claim 1 , wherein the formaldehyde donor comprises imidazolidinyl urea, diazolidinyl urea, dimethylol-5,5-dimethylhydantoin, dimethylol urea, 2-bromo-2-nitropropane-1,3-diol, 1,3-bis(hydroxymethyl)-5,5-dimethylimidazolidine-2,4-dione (DMDM-hydantoin), N5-methyl-tetrahydrofolic acid, N10-methyl-tetrahydrofolic acid or a mixture thereof.
5 . The method according to claim 8 , wherein the liquid is a buffer, physiological common salt solution or the like.
6 . The method according to claim 1 , wherein the biological cell is separated by means of antibodies or via other conventional methods.
7 . A method for the detection of a tumor cell comprising preventing time-dependent expression of mRNA in a biological cell within a sample comprising mixing the sample with a reagent comprising a formaldehyde donor.
8 . The method of claim 7 , wherein in the sample is a body fluid, blood, liquor, ascites, urine, a tissue piece or a suspension which contains cells from tissue sample.
9 . The method of claim 7 , wherein said reagent further comprises a fluid.
10 . The method of claim 1 , wherein said reagent further comprises a fluid.