Biological dataset profiling of cardiovascular disease and cardiovascular inflammation
View Patent ↗Methods and systems for evaluating biological dataset profiles relating to inflammatory cardiovascular conditions are provided, where datasets comprising information for multiple cellular parameters are compared and identified, and used in the evaluation of candidate pharmacologic agents for suitability as therapeutic agents.
1. A method comprising:
contacting an agent with two or more cell culture systems selected from the group consisting of:
(a) human neonatal fibroblasts (HDFn) in the presence of TNF, IFNγ and TGFβ, and measuring the levels of at least three different proteins selected from the group consisting of: ICAM, VCAM, CD40, CD90, IP-10, MCP-1, Collagen I, Mig, m-CSF, TIMP-2, PAI-I, and IL-8, prior to and subsequent to the contacting;
(b) HDFn in the presence of TGFβ, and measuring the levels of at least three different proteins selected from the group consisting of: CD90, Collagen I, Collagen III, HLA-DR, PAI-I, and VCAM, prior to and subsequent to the contacting;
(c) primary human artery smooth muscle cells in the presence of TNF-alpha, IL-1, and IFNγ, and measuring the levels of at least three different proteins selected from the group consisting of: VCAM, CD40, HLA-DR, ICAM, IL-8, MCP-1, M-CSF, MIG, Thrombomodulin, serum amyloid A, and uPAR, prior to and subsequent to the contacting; and
(d) macrophages and endothelial cells cultured in the presence of Toll-like receptor ligands, and measuring the levels of at least three different proteins selected from the group consisting of: CD38, CD40, CD69, E-selectin, IL-8, MCP-1, and MIG, prior to and subsequent to the contacting; and
determining changes in protein expression that result from the contacting of said agent, wherein the determining is based on differences between the measured protein levels prior to and subsequent to the contacting;
recording said changes in protein expression.
2. The method of claim 1 further comprising simultaneous analysis of three or four of cell culture system (a), cell culture system (b), cell culture system (c), and cell culture system (d).
3. The method of claim 1 further comprising simultaneous analysis of each of cell culture system (a), cell culture system (b), cell culture system (c), and cell culture system (d).
4. The method according to claim 1 , wherein said agent is a genetic agent.
5. The method according to claim 1 , wherein said agent is a chemical or biological agent.
6. The method according to claim 1 , wherein a plurality of cell culture systems are concatenated for simultaneous analysis.