L-arabinose fermenting yeast
View Patent ↗An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.
1. An isolated polynucleotide encoding an isolated non-conventional yeast arabinose transporter comprising the capability of adapting a conventional yeast for growth on arabinose when the arabinose transporter is included in the conventional yeast, wherein the transporter comprises an amino acid sequence at least 95% identical to SEQ ID NO:2.
2. The isolated polynucleotide of claim 1 , wherein the polynucleotide comprises SEQ ID NO:1.
3. A vector comprising the polynucleotide of claim 1 .
4. A yeast cell comprising the vector of claim 3 .
5. The isolated polynucleotide of claim 1 , wherein the transporter comprises the amino acid sequence of SEQ ID NO:2.