Compositions and methods for predicting outcome of treatment
View Patent ↗This invention is directed to compositions and their uses for detection of markers. Such markers may be useful in the understanding of the underlying molecular event leading to a condition or a disease in a subject. These markers may also be useful for characterization of neoplastic cells and cancer cells and their response to certain therapeutical regimes. Therefore the invention as disclosed may contribute to the improvement of the stratification of patients for the best possible treatment.
1. A method for predicting the outcome of 5-FU treatment in a subject having a breast cancer, wherein said subject has not previously undergone 5-FU treatment, the method comprising the steps:
a) determining the gene copy number of each of at least two markers from the 5-FU pathway in an isolated breast cancer sample from said subject, wherein the at least two markers are TYMS and DHFR,
b) detecting a decreased copy number of at least one of the markers, and
c) predicting the outcome of the 5-FU treatment in said subject based on the gene copy number levels detected, wherein a decrease in the gene copy number for either TYMS or DHFR in the breast cancer sample is predictive of breast cancer resistance to 5-FU treatment; and wherein the decrease in TYMS and DHFR gene copy number is measured with reference to at least one reference marker, wherein the at least one reference marker comprises a centromere probe.
2. The method according to claim 1 , further comprising detecting a signal from at least one reference marker in the centromere region of the same chromosome as the TYMS or DHFR gene, and determining the decrease in TYMS or DHFR gene copy number based on correlation of the signal from the TYMS or DHFR gene and the signal from the at least one reference marker.
3. The method of claim 2 , wherein the gene copy number is determined using probes, wherein the probes are chosen from nucleic acid probes and nucleic acid analogue probes.
4. The method of claim 2 , wherein the at least one reference marker is in a centromere chosen from Cen5 and Cen18.
5. The method of claim 2 further comprising using at least one blocking probe.