Monoclonal antibodies recognizing a coagulase-negative
Monoclonal antibodies which can bind to the SdrF protein of Staphylococcus epidermidis are provided which can be useful in the treatment and protection against infection from staphylococcal bacteria such as Staphylococcus epidermidis . The monoclonal antibodies of the invention are advantageous in that they can also recognize binding domains and subdomains of the S. epidermidis SdrF protein in addition to the protein itself. Suitable compositions and passive vaccines based on the monoclonal antibodies of the invention, as well as methods for their use, are also provided.
1. An isolated monoclonal antibody which binds specifically to the SdrF protein of Staphylococcus epidermidis and which has a variable light chain selected from the group consisting of SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 24, SEQ ID NO: 27, SEQ ID NO: 29 and SEQ ID NO: 31.
2. The monoclonal antibody according to claim 1 wherein the antibody is raised against the N1N2N3 subregion of the SdrF protein.
3. The monoclonal antibody according to claim 1 wherein the monoclonal antibody is raised against a peptide selected from the group consisting of an antigenic subregion of the SdrF protein, the SdrF ligand binding A domain, the N1 subregion, the N2 subregion, the N3 subregion, and combinations of said subregions.
4. The monoclonal antibody according to claim 1 , wherein said antibody is suitable for parenteral, oral, intranasal, subcutaneous, aerosolized or intravenous administration in a human or animal.
5. The monoclonal antibody according to claim 1 wherein the monoclonal antibody is selected from the group consisting of murine, chimeric, humanized and human monoclonal antibodies.
6. The monoclonal antibody according to claim 1 wherein the antibody is a single chain monoclonal antibody.
7. The monoclonal antibody according to claim 1 that is raised against a peptide having the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:12, and SEQ ID NO:14.
8. The monoclonal antibody according to claim 1 wherein the monoclonal antibody recognizes a peptide selected from the group consisting of an antigenic subregion of the SdrF protein, the SdrF ligand binding A domain, the N1 subregion, the N2 subregion, the N3 subregion, and combinations of said subregions.
9. The monoclonal antibody according to claim 1 that recognizes a peptide having the amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8 and SEQ ID NO:10 and SEQ ID NO:12, and SEQ ID NO:14.
10. Isolated antisera containing the antibody according to claim 1 .
11. A pharmaceutical composition comprising an effective amount of the antibody of claim 1 and a pharmaceutically acceptable vehicle, carrier or excipient.
12. The pharmaceutical composition according to claim 11 further comprising a physiologically acceptable antibiotic.
13. An isolated monoclonal antibody which binds specifically to the SdrF protein of S. epidermidis , wherein said antibody has a variable heavy chain selected from the group consisting of SEQ ID NO: 18, SEQ ID NO: 22, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO: 30, SEQ ID NO: 32 and SEQ ID NO: 34.
14. A method of detecting S. epidermidis in a sample from a patient infected with S. epidermidis comprising adding the monoclonal antibody of claim 1 to the sample and determining the specific binding of the antibody.
15. A method of making the monoclonal antibody of claim 1 comprising administering to a host animal an immunogenic amount of a peptide selected from the group consisting of an antigenic subregion of the SdrF protein, the SdrF ligand binding A domain, the N1 subregion, the N2 subregion, the N3 subregion, and combinations of said subregions, forming a hybridoma, and isolating the monoclonal antibody from said hybridoma.
16. A kit comprising the monoclonal antibody of claim 1 and means for detecting specific binding by said antibody.
17. The kit of claim 16 , wherein said means for detecting the binding comprises a detectable label that is linked to said antibody.