Methods for increasing the resistance of plants to hypoxic conditions
View Patent ↗Methods are provided for increasing the resistance of plants to hypoxic or anoxic conditions. Such methods may be applied to increase the penetrance of plant roots in the growth medium or into soil. The methods according to the invention may include providing plants with a stress tolerance gene. Similar effects can be obtained by applying chemical compounds, including neonicotinoid compounds, to the plants.
1. A method for increasing the tolerance of a plant cell or plant to hypoxic or anoxic conditions, comprising:
a) applying an effective amount of a compound of formula (I)
wherein
Het represents a heterocycle which is either mono- or polysubstituted by fluorine, chlorine, methyl or ethyl, wherein said heterocycle is:
pyrid-3-yl, pyrid-5-yl, 3-pyridinio, 1-oxido-5-pyridinio, 1-oxido-5-pyridinio, tetra-hydrofuran-3-yl, or thiazol-5-yl,
A represents C 1 -C 6 -alkyl, —N(R 1 )(R 2 ) or S(R 2 ),
in which
R 1 represents hydrogen, C 1 -C 6 -alkyl, phenyl-C 1 -C 4 -alkyl, C 3 -C 6 -cycloalkyl, C 2 -C 6 -alkenyl or C 2 -C 6 -alkynyl, and
R 2 represents C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl, C 2 -C 6 -alkynyl, —C(═O)—CH 3 or benzyl,
R represents hydrogen, C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl, C 2 -C 6 -alkynyl, —C(═O)—CH 3 or benzyl or together with R 2 represents the groups below:
—CH 2 —CH 2 —, CH 2 —CH 2 —CH 2 —, —CH 2 —O—CH 2 —, —CH 2 —S—CH 2 —, —CH 2 —NH—CH 2 —, —CH 2 —N(CH 3 )—CH 2 —, and
X represents N—NO 2 , N—CN or CH—NO 2
on said plant cell or plant or on its locus, or on seeds of said plant; and
b) identifying a plant or plant cell which has an increased tolerance to hypoxic or anoxic conditions compared to a plant cell or plant where said compound was not applied to the plant, its locus or its seeds.
2. A method for increasing the penetrance of roots of a plant into a growth medium, comprising:
a) applying an effective amount of a compound of formula (I)
wherein
Het represents a heterocycle which is either mono- or polysubstituted by fluorine, chlorine, methyl or ethyl, wherein said heterocycle is:
pyrid-3-yl, pyrid-5-yl, 3-pyridinio, 1-oxido-5-pyridinio, 1-oxido-5-pyridinio, tetra-hydrofuran-3-yl, or thiazol-5-yl,
A represents C 1 -C 6 -alkyl, —N(R 1 )(R 2 ) or S(R 2 ),
in which
R 1 represents hydrogen, C 1 -C 6 -alkyl, phenyl-C 1 -C 4 -alkyl, C 3 -C 6 -cycloalkyl, C 2 -C 6 -alkenyl or C 2 -C 6 -alkynyl, and
R 2 represents C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl, C 2 -C 6 -alkynyl, —C(═O)—CH 3 or benzyl,
R represents hydrogen, C 1 -C 6 -alkyl, C 2 -C 6 -alkenyl, C 2 -C 6 -alkynyl, —C(═O)—CH 3 or benzyl or together with R 2 represents the groups below:
—CH 2 —CH 2 —, —CH 2 —CH 2 —CH 2 —, —CH 2 —O—CH 2 —, —CH 2 —S—CH 2 —, —CH 2 —NH—CH 2 —, —CH 2 —N(CH 3 )—CH 2 —, and
X represents N—NO 2 , N—CN or CH—NO 2
on said plant or on its locus, or on seeds of said plant; and
b) identifying a plant which has an increased penetrance of roots into a growth medium compared to a plant where said compound was not applied to the plant, its locus or its seeds.
3. The method of claim 1 or 2 , wherein said compound of formula (I) is a neonicotinoid compound.
4. The method of claim 1 or 2 , wherein said heterocycle represented by Het of said compound of formula (I) is a pyrid-3-yl heterocycle substituted by chlorine.
5. The method of claim 4 , wherein said compound of formula (I) is imidacloprid, or thiacloprid.
6. A method for increasing the tolerance of a plant to hypoxic or anoxic conditions, comprising the step of: providing said plant with an effective amount of 6-chloronicotinic acid, whereby said 6-chloronicotinic acid is applied directly to said plant or the habitat thereof, or said plant's seed, and the tolerance of said plant to hypoxic or anoxic conditions is increased.
7. A method for increasing the penetrance of roots of a plant into a growth medium comprising the step of: providing said plant with an effective amount of 6-chloronicotinic acid, whereby said 6-chloronicotinic acid is applied directly to said plant or the habitat thereof, or said plant's seed, and the penetrance of roots of said plant into a growth medium is increased.
8. The method of claim 1 or 2 , wherein said compound is applied to said plant, said locus, or said seeds when planting said plant or seed.