Hemoglobin derivative measurement method, and reagent composition, measurement kit, analysis device and analysis system for use in the method
View Patent ↗A sample solution including blood components is processed with a denaturalization reagent comprising a nonionic surface-activating agent and an oxidizing agent to denaturalize a hemoglobin derivative in the sample solution, and thereafter, an immunoassay is performing utilizing an antibody that is specific to a denaturalized site of the hemoglobin derivative to measure the amount of the hemoglobin derivative in the sample. Therefore, when performing assay of hemoglobin derivative, denaturalization of hemoglobin can be performed speedily and reliably while minimizing adverse effects of the denaturalization reagent on immune reaction.
1. A hemoglobin derivative measurement method comprising:
processing a sample which comprises blood components including erythrocytes, with a reagent composition comprising:
an oxidizing agent, and
a nonionic surface-activating agent selected from the group consisting of n-Decyl-β-D-maltoside, n-Nonyl-β-D-thiomaltoside, Sucrose monocaprate, and Sucrose monolaurate,
whereby the combination of said nonionic surface-activating agent with the oxidizing agent denaturalizes a hemoglobin derivative from the erythrocytes in the sample; and
detecting the denatured hemoglobin derivative in the sample by performing an immunoassay using an antibody that is specific to a denaturalized site of the hemoglobin derivative.
2. A hemoglobin derivative measurement method as defined in claim 1 wherein said hemoglobin derivative is glycated hemoglobin, and said antibody is specific to a denaturalized site of the glycated hemoglobin.
3. A hemoglobin derivative measurement method as defined in claim 1 further including measuring the hemoglobin included in the sample, and calculating an abundance ratio of the hemoglobin derivative to the hemoglobin.
4. A hemoglobin derivative measurement method as defined in claim 3 wherein said hemoglobin derivative is glycated hemoglobin.
5. A reagent composition for measuring a hemoglobin derivative in a sample which comprises blood components including erythrocytes, said reagent composition comprising:
a nonionic surface-activating agent selected from the group consisting of n-Decyl-β-D-maltoside, n-Nonyl-β-D-thiomaltoside, Sucrose monocaprate, and Sucrose monolaurate;
an oxidizing agent; and
an antibody that is specific to a denaturalized site of the hemoglobin derivative from the erythrocytes.
6. A reagent composition as defined in claim 5 wherein said hemoglobin derivative is glycated hemoglobin, and said antibody is specific to a denaturalized site of the glycated hemoglobin.
7. A measurement kit for measuring a hemoglobin derivative in a sample which comprises blood components including erythrocytes, said measurement kit holding:
a reagent composition comprising:
a nonionic surface-activating agent selected from the group consisting of n-Decyl-β-D-maltoside, n-Nonyl-β-D-thiomaltoside, Sucrose monocaprate, and Sucrose monolaurate; and
an oxidizing agent;
and an antibody that is specific to a denaturalized site of a hemoglobin derivative from the erythrocytes.
8. A measurement kit as defined in claim 7 wherein said hemoglobin derivative is glycated hemoglobin, and said antibody is specific to a denaturalized site of the glycated hemoglobin.