Elastin protective polyphenolics and methods of using the same
View Patent ↗Dermal fibroblasts permanently loose their ability to synthesize elastin, the major component of elastic fibers, shortly after puberty. This progressive loss of elastic fibers cannot be replaced, resulting in the physical signs of aging. The present invention provides methods and compositions containing the polyphenols ellagic acid and/or tannic acid for protection against degradation of cutaneous elastic fibers by the elastolytic enzymes. The use of ellagic acid and/or tannic acid increased the overall deposition of elastic fibers in healthy and damaged skin cells. The protection of both intra-tropoelastin and extra-cellular mature elastic fibers from proteolytic enzymes by ellagic acid and tannic acid caused an increase in the net deposition of elastic fibers. Therefore, embodiments of the present invention provide methods and composition for the treatment of skin and prevention and treatment of degradation of dermal elastic fibers.
1. A method for stabilizing elastin in skin of a subject comprising topically administering to the skin in need thereof a composition comprising from about 1 μg to about 10 μg of a polyphenolic compound, wherein the elastin is stabilized.
2. The method of claim 1 , wherein said polyphenolic compound is selected from tannic acid, ellagic acid and combinations thereof.
3. The method of claim 1 further comprising administering a stimulator of elastogenesis selected from elastin-derived peptides, plant-derived peptides, bovine-derived peptides, manganese, iron, copper and combinations thereof.
4. The method of claim 3 , wherein said composition and said stimulator of elastogenesis are administered simultaneously.
5. The method of claim 3 , wherein said composition and said stimulator of elastogenesis are administered sequentially.
6. The method of claim 1 further comprising administering a peptide selected from the group consisting of SEQ ID NO: 1 (Val-Gly-Ala-Met-Pro-Gly), SEQ ID NO: 2 (Val-Gly-Leu-Met-Pro-Gly), SEQ ID NO: 3 (Val-Gly-Val-Met-Pro-Gly), SEQ ID NO: 4 (Val-Gly-Ala-Ser-Pro-Gly), SEQ ID NO: 5 (Val-Gly-Ala-Ala-Pro-Gly), SEQ ID NO: 6 (Val-Gly-Leu-Ser-Pro-Gly), SEQ ID NO: 7 (Val-Gly-Leu-Ala-Pro-Gly), SEQ ID NO: 8 (Val-Gly-Val-Ser-Pro-Gly), SEQ ID NO: 9 (Ile-Gly-Ala-Met-Pro-Gly), SEQ ID NO: 10 (Ile-Gly-Leu-Met-Pro-Gly), SEQ ID NO: 11 (Ile-Gly-Val-Met-Pro-Gly), SEQ ID NO: 12 (Ile-Gly-Ala-Ser-Pro-Gly), SEQ ID NO: 13 (Ile-Gly-Ala-Ala-Pro-Gly), SEQ ID NO: 14 (Ile-Gly-Leu-Ser-Pro-Gly), SEQ ID NO: 15 (Ile-Gly-Leu-Ala-Pro-Gly), SEQ ID NO: 16 (Ile-Gly-Val-Ser-Pro-Gly), SEQ ID NO: 17 (Ile-Gly-Val-Ala-Pro-Gly) and combinations thereof.
7. The method of claim 6 , wherein said polyphenolic compound and said peptide are administered simultaneously.
8. The method of claim 6 , wherein said polyphenolic compound and said peptide are administered sequentially.
9. The method of claim 1 , wherein the deposition of insoluble elastin fibers is increased.
10. The method of claim 1 , wherein the skin is wrinkled.
11. The method of claim 10 , wherein the wrinkling of the skin is reduced.
12. The method of claim 1 , wherein said effective amount of said polyphenolic compound is about 1 μg.
13. A method of treating damage to the skin of a subject comprising topically administering to the skin in need thereof a composition comprising from about 1 μg to about 10 μg of a polyphenolic compound, wherein the deposition of insoluble elastin fibers is increased.
14. The method of claim 13 , wherein the damage to the skin is wrinkles.
15. The method of claim 14 , wherein the wrinkling of the skin is reduced.
16. The method of claim 13 , wherein said polyphenolic compound is selected from tannic acid, ellagic acid and combinations thereof.
17. The method of claim 13 , wherein said effective amount of said polyphenolic compound is about 1 μg.
18. The method of claim 13 further comprising administering a stimulator of elastogenesis selected from elastin-derived peptides, plant-derived peptides, bovine-derived peptides, manganese, iron, copper and combinations thereof.
19. The method of claim 18 , wherein said composition and said stimulator of elastogenesis are administered simultaneously.
20. The method of claim 18 , wherein said composition_and said stimulator of elastogenesis are administered sequentially.
21. The method of claim 13 further comprising administering a peptide selected from the group consisting of SEQ ID NO: 1 (Val-Gly-Ala-Met-Pro-Gly), SEQ ID NO: 2 (Val-Gly-Leu-Met-Pro-Gly), SEQ ID NO: 3 (Val-Gly-Val-Met-Pro-Gly), SEQ ID NO: 4 (Val-Gly-Ala-Ser-Pro-Gly), SEQ ID NO: 5 (Val-Gly-Ala-Ala-Pro-Gly), SEQ ID NO: 6 (Val-Gly-Leu-Ser-Pro-Gly), SEQ ID NO: 7 (Val-Gly-Leu-Ala-Pro-Gly), SEQ ID NO: 8 (Val-Gly-Val-Ser-Pro-Gly), SEQ ID NO: 9 (Ile-Gly-Ala-Met-Pro-Gly), SEQ ID NO: 10 (Ile-Gly-Leu-Met-Pro-Gly), SEQ ID NO: 11 (Ile-Gly-Val-Met-Pro-Gly), SEQ ID NO: 12 (Ile-Gly-Ala-Ser-Pro-Gly), SEQ ID NO: 13 (Ile-Gly-Ala-Ala-Pro-Gly), SEQ ID NO: 14 (Ile-Gly-Leu-Ser-Pro-Gly), SEQ ID NO: 15 (Ile-Gly-Leu-Ala-Pro-Gly), SEQ ID NO: 16 (Ile-Gly-Val-Ser-Pro-Gly), SEQ ID NO: 17 (Ile-Gly-Val-Ala-Pro-Gly) and combinations thereof.
22. The method of claim 21 , wherein said polyphenolic compound and said peptide are administered simultaneously.
23. The method of claim 21 , wherein said polyphenolic compound and said peptide are administered sequentially.