METHOD FOR BLOCKING NON-SPECIFIC PROTEIN BINDING ON A FUNCTIONALIZED SURFACE
A method of blocking non-specific protein binding on surfaces, such as protein-coated biosensor surfaces.
1 . A method for reducing non-specific binding on a surface, wherein the surface is aldehyde-functionalized, amine-functionalized, or a combination thereof, comprising:
treating the surface with sugar molecules, whereby non-specific binding on the surface is reduced.
2 . The method of claim 1 , wherein the sugar molecules comprise disaccharides.
3 . The method of claim 2 , wherein the disaccharides are trehalose molecules or comprise trehalose molecules.
4 . The method of claim 1 , wherein the sugar molecules are dextran sulfate or comprise dextran sulfate.
5 . The method of claim 1 , wherein the surface is a biosensor surface.
6 . The method of claim 5 , wherein the biosensor is a calorimetric resonant reflectance biosensor.
7 . The method of claim 1 , wherein the sugar molecules comprise monosaccharides, disaccharides, polysaccharides, trisaccharides, tetrasaccharides, pentasaccharides, or a combination thereof.
8 . The method of claim 1 , wherein the surface is an amine-functionalized surface.
9 . The method of claim 8 , wherein the sugar molecules comprises lactose, glyceraldehydes, or a combination thereof.
10 . The method of claim 1 , wherein the sugar molecules comprise trehalose and lactose.
11 . The method of claim 1 , wherein the sugar molecules comprise trehalose and glyceraldehyde.
12 . A biosensor comprising a plurality of specific binding substances bound to surface-attached aldehyde groups and a plurality of sugar molecules bound to surface-attached aldehyde groups.
13 . The biosensor of claim 12 , wherein the sugar molecules comprise disaccharides.
14 . The biosensor of claim 13 , wherein the disaccharides are trehalose molecules.
15 . The biosensor of claim 15 , wherein the sugar molecules are disaccharides.
16 . The biosensor of claim 13 , wherein the disaccharides are trehalose molecules.
17 . The biosensor of claim 12 , wherein the sugar molecules comprise dextran.
18 . The biosensor of claim 12 , wherein the sugar molecules are dextran.
19 . The biosensor of claim 12 , wherein the specific binding substances are proteins.
20 . The biosensor of claim 19 , wherein the proteins comprise streptavidin.
21 . A package containing a biosensor with specific binding substances bound to surface-attached aldehyde groups and a storage solution comprising sugar molecules.
22 . The package of claim 21 , wherein the sugar molecules comprise disaccharides.
23 . The package of claim 22 , wherein the disaccharides comprise trehalose.
24 . The package of claim 21 , wherein the sugar molecules comprise dextran.
25 . The package of claim 21 , wherein the sugar molecules are dextran.