Method of targeting specific cell populations using cell-binding agent maytansinoid conjugates linked via a non-cleavable linker, said conjugates and methods of making said conjugates
The present invention discloses a method for targeting maytansinoids to a selected cell population, the method comprising contacting a cell population or tissue suspected of containing the selected cell population with a cell-binding agent maytansinoid conjugate, wherein one or more maytansinoids is covalently linked to the cell-binding agent via a non-cleavable linker and the cell-binding agent binds to cells of the selected cell population.
1. A compound of formula 5:
wherein X is a linear or branched alkyl, alkenyl or alkynyl group bearing 2 to 20 carbon atoms, Y is a cycloalkyl or cycloalkenyl group bearing 3 to 10 carbon atoms, Z is an aromatic group having 6 to 10 carbon atoms and optionally substituted with at least one alkyl substituent containing from 1 to 4 carbon atoms, alkoxy substituent containing one or two carbon atoms, halogen or nitro group or a heterocyclic group having a 3 to 10-membered ring system containing one or two heteroatoms selected from N, O or S and optionally substituted with one or two nitro group, and l, m and n are each 0 or 1, provided that they are all not 0 at the same time, and E together with the adjacent carbonyl group forms an activated ester.
2. A compound of formula 6:
wherein n′ represents an integer from 3 to 24, and E together with the adjacent carbonyl group forms an activated ester.
3. A compound of formula 7:
wherein R is H or SO 3 − Na + .
4. The compound of claim 1 , wherein the activated ester is an N-hydroxy succinimidyl ester, an N-hydroxy sulfosuccinimidyl ester, an N-hydroxy phthalimidyl ester, an N-hydroxy sulfophthalimidyl ester, an ortho-nitrophenyl ester, a para-nitrophenyl ester, a 2,4-dinitrophenyl ester, a 3-sulfonyl-4-nitrophenyl ester, a 3-carboxy-4-nitrophenyl ester, a pentafluorophenyl ester, or a sulfonyl tetrafluorophenyl ester.
5. The compound of claim 2 , wherein the activated ester is an N-hydroxy succinimidyl ester, an N-hydroxy sulfosuccinimidyl ester, an N-hydroxy phthalimidyl ester, an N -hydroxy sulfophthalimidyl ester, an ortho-nitrophenyl ester, a para-nitrophenyl ester, a 2,4-dinitrophenyl ester, a 3-sulfonyl-4-nitrophenyl ester, a 3-carboxy-4-nitrophenyl ester, a pentafluorophenyl ester, or a sulfonyl tetrafluorophenyl ester.