IP Library Granted Patent US 8,609,935
Granted Patent B2
US 8,609,935 · App. 11/927,884 · Granted Dec 17, 2013

Soybean event DP-305423-1 and compositions and methods for the identification and/or detection thereof

Inventors: Anthony J. Kinney (Wilmington, DE); Keven L. Stecca (New Castle, DE); Knut Meyer (Wilmington, DE); Kent Brink (Newark, DE); Robert F. Cressman, Jr. (Wilmington, DE); Natalie N. Weber (Hockessin, DE); Cathy Xiaoyan Zhong (Wilmington, DE)
Assignee: E. I. du Pont de Nemours and Company
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Quick Facts
Patent No.
US 8,609,935
App. No.
11/927,884
Granted
Dec 17, 2013
Kind
B2
Abstract

Compositions and methods related to transgenic high oleic acid/ALS inhibitor-tolerant soybean plants are provided. Specifically, the present invention provides soybean plants having a DP-305423-1 event which imparts a high oleic acid phenotype and tolerance to at least one ALS-inhibiting herbicide. The soybean plant harboring the DP-305423-1 event comprises genomic/transgene junctions having at least the polynucleotide sequence of SEQ ID NO:8, 9, 14, 15, 20, 21, 83 or 84. The characterization of the genomic insertion site of the DP-305423-1 event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the soybean DP-305423-1 events are provided.

Claims (11)

1. A soybean plant comprising nucleotides 18,652-31,579 of SEQ ID NO:5.

2. A soybean seed comprising nucleotides 18,652-31,579 of SEQ ID NO:5.

3. The soybean plant of claim 1 , further comprising nucleotides 12,164-14,494 of SEQ ID NO:6, nucleotides 5751-7813 of SEQ ID NO:7 and nucleotides 2899-7909 of SEQ ID NO:82.

4. The soybean seed of claim 2 , wherein said seed has an oleic acid level of greater than 70%.

5. The soybean seed of claim 2 , further comprising nucleotides 12,164-14,494 of SEQ ID NO:6, nucleotides 5751-7813 of SEQ ID NO:7 and nucleotides 2899-7909 of SEQ ID NO:82.

6. The soybean seed of claim 5 , wherein said seed has an oleic acid level of greater than 70%.

7. A method of producing an ALS inhibitor tolerant plant comprising breeding a plant comprising a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO:5, and selecting progeny by analyzing for progeny that comprise a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO: 5.

8. A method for controlling weeds in an area of cultivation comprising applying an effective amount of an ALS inhibitor to the area of cultivation comprising soybean plants comprising a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO:5.

9. The method of claim 8 further comprising applying an effective amount of at least one herbicide selected from the group consisting of inhibitors of Acetyl CoA carboxylase, inhibitors of Photosystem II, Photosystem I electron diverters, inhibitors of PPO, inhibitors of carotenoid biosynthesis, inhibitors of 4-HPPD, inhibitors of DHP, inhibitors of EPSP synthase, inhibitors of glutamine synthetase, inhibitors of microtubule assembly, inhibitors of mitosis/microtubule organization, inhibitors of cell division, inhibitors of cell wall synthesis, membrane disruptors, inhibitors of lipid synthesis by other than ACC inhibition, synthetic auxins, inhibitors of auxin transport, and Other Mechanism of Action herbicides, as described in Table 2.

10. A method for controlling weeds in an area of cultivation comprising applying an effective amount of at least one herbicide selected from the group consisting of acetochlor, acifluorfen and its sodium salt, aclonifen, acrolein (2-propenal), alachlor, alloxydim, ametryn, amicarbazone, amidosulfuron, aminopyralid, amitrole, ammonium sulfamate, anilofos, asulam, atrazine, azimsulfuron, beflubutamid, benazolin, benazolin-ethyl, bencarbazone, benfluralin, benfuresate, bensulfuron-methyl, bensulide, bentazone, benzobicyclon, benzofenap, bifenox, bilanafos, bispyribac and its sodium salt, bromacil, bromobutide, bromofenoxim, bromoxynil, bromoxynil octanoate, butachlor, butafenacil, butamifos, butralin, butroxydim, butylate, cafenstrole, carbetamide, carfentrazone-ethyl, catechin, chlomethoxyfen, chloramben, chlorbromuron, chlorflurenol-methyl, chloridazon, chlorimuron-ethyl, chlorotoluron, chlorpropham, chlorsulfuron, chlorthal-dimethyl, chlorthiamid, cinidon-ethyl, cinmethylin, cinosulfuron, clethodim, clodinafop-ropargyl, clomazone, clomeprop, clopyralid, clopyralid-olamine, cloransulam-methyl, CUH-35 (2-methoxyethyl 2-[[[4-chloro-2-fluoro-5-[(1-methyl-2-propynyl)-oxy]-phenyl](3-fluoro-benzoyl)-amino]carbonyl]-1-cyclohexene-1-carboxylate), cumyluron, cyanazine, cycloate, cyclosulfamuron, cycloxydim, cyhalofop-butyl, 2,4-D and its butotyl, butyl, isoctyl and isopropyl esters and its dimethylammonium, diolamine and trolamine salts, daimuron, dalapon, dalapon-sodium, dazomet, 2,4-DB and its dimethylammonium, potassium and sodium salts, desmedipham, desmetryn, dicamba and its diglycolammonium, dimethylammonium, potassium and sodium salts, dichlobenil, dichlorprop, diclofop-methyl, diclosulam, difenzoquat metilsulfate, diflufenican, diflufenzopyr, dimefuron, dimepiperate, dimethachlor, dimethametryn, dimethenamid, dimethenamid-P, dimethipin, dimethylarsinic acid and its sodium salt, dinitramine, dinoterb, diphenamid, diquat dibromide, dithiopyr, diuron, DNOC, endothal, EPTC, esprocarb, ethalfluralin, ethametsulfuron-methyl, ethofumesate, ethoxyfen, ethoxysulfuron, etobenzanid, fenoxaprop-ethyl, fenoxaprop-P-ethyl, fentrazamide, fenuron, fenuron-TCA, flamprop-methyl, flamprop-M-isopropyl, flamprop-M-methyl, flazasulfuron, florasulam, fluazifop-butyl, fluazifop-P-butyl, flucarbazone, flucetosulfuron, fluchloralin, flufenacet, flufenpyr, flufenpyr-ethyl, flumetsulam, flumiclorac-pentyl, flumioxazin, fluometuron, fluoroglycofen-ethyl, flupyrsulfuron-methyl and its sodium salt, flurenol, flurenol-butyl, fluridone, flurochloridone, fluroxypyr, flurtamone, fluthiacet-methyl, fomesafen, foramsulfuron, fosamine-ammonium, glufosinate, glufosinate-ammonium, glyphosate and its salts such as ammonium, isopropylammonium, potassium, sodium (including sesquisodium) and trimesium (alternatively named sulfosate), halosulfuron-methyl, haloxyfop-etotyl, haloxyfop-methyl, hexazinone, HOK-201 (N-(2,4-difluorophenyl)-1,5-dihydro-N-(1-methylethyl)-5-oxo-1-[(tetrahydro-2H-pyran-2-ylymethyl]-4H-1,2,4-triazole-4-carboxamide), imazamethabenz-methyl, imazamox, imazapic, imazapyr, imazaquin, imazaquin-ammonium, imazethapyr, imazethapyr-ammonium, imazosulfuron, indanofan, iodosulfuron-methyl, ioxynil, ioxynil octanoate, ioxynil-sodium, isoproturon, isouron, isoxaben, isoxaflutole, isoxachlortole, lactofen, lenacil, linuron, maleic hydrazide, MCPA and its salts (e.g., MCPA-dimethylammonium, MCPA-potassium and MCPA-sodium, esters (e.g., MCPA-2-ethylhexyl, MCPA-butotyl) and thioesters (e.g., MCPA-thioethyl), MCPB and its salts (e.g., MCPB-sodium) and esters (e.g., MCPB-ethyl), mecoprop, mecoprop-P, mefenacet, mefluidide, mesosulfuron-methyl, mesotrione, metam-sodium, metamifop, metamitron, metazachlor, methabenzthiazuron, methylarsonic acid and its calcium, monoammonium, monosodium and disodium salts, methyldymron, metobenzuron, metobromuron, metolachlor, S-metholachlor, metosulam, metoxuron, metribuzin, metsulfuron-methyl, molinate, monolinuron, naproanilide, napropamide, naptalam, neburon, nicosulfuron, norflurazon, orbencarb, oryzalin, oxadiargyl, oxadiazon, oxasulfuron, oxaziclomefone, oxyfluorfen, paraquat dichloride, pebulate, pelargonic acid, pendimethalin, penoxsulam, pentanochlor, pentoxazone, perfluidone, pethoxyamid, phenmedipham, picloram, picloram-potassium, picolinafen, pinoxaden, piperofos, pretilachlor, primisulfuron-methyl, prodiamine, profoxydim, prometon, prometryn, propachlor, propanil, propaquizafop, propazine, propham, propisochlor, propoxycarbazone, propyzamide, prosulfocarb, prosulfuron, pyraclonil, pyraflufen-ethyl, pyrasulfotole, pyrazogyl, pyrazolynate, pyrazoxyfen, pyrazosulfuron-ethyl, pyribenzoxim, pyributicarb, pyridate, pyriftalid, pyriminobac-methyl, pyrimisulfan, pyrithiobac, pyrithiobac-sodium, pyroxsulam, quinclorac, quinmerac, quinoclamine, quizalofop-ethyl, quizalofop-P-ethyl, quizalofop-P-tefuryl, rimsulfuron, sethoxydim, siduron, simazine, simetryn, sulcotrione, sulfentrazone, sulfometuron-methyl, sulfosulfuron, 2,3,6-TBA, TCA, TCA-sodium, tebutam, tebuthiuron, tefuryltrione, tembotrione, tepraloxydim, terbacil, terbumeton, terbuthylazine, terbutryn, thenylchlor, thiazopyr, thiencarbazone, thifensulfuron-methyl, thiobencarb, tiocarbazil, topramezone, tralkoxydim, tri-allate, triasulfuron, triaziflam, tribenuron-methyl, triclopyr, triclopyr-butotyl, triclopyr-triethylammonium, tridiphane, trietazine, trifloxysulfuron, trifluralin, triflusulfuron-methyl, tritosulfuron and vernolate to the area of cultivation comprising soybean plants comprising a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO:5.

11. A method for controlling weeds in an area of cultivation comprising applying an effective amount of at least one sulfonylurea herbicide and at least one imidazolinone herbicide to the area of cultivation comprising soybean plants comprising a polynucleotide comprising nucleotides 18,652-31,579 of SEQ ID NO:5.

Assignments (3)
NUNC PRO TUNC ASSIGNMENT Recorded Nov 29, 2022
From: E.I. DU PONT DE NEMOURS AND COMPANY
To: CORTEVA AGRISCIENCE LLC
Reel/Frame 063141/0155 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 9, 2017
From: BRINK, KENT; WEBER, NATALIE N; ZHONG, CATHY XIAOYAN; CRESSMAN, ROBERT F., JR.
To: E. I. DU PONT DE NEMOURS AND COMPANY
Reel/Frame 043243/0215 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 6, 2010
From: KINNEY, ANTHONY J.; STECCA, KEVIN L.; MEYER, KNUT
To: E.I. DU PONT DE NEMOURS AND COMPANY
Reel/Frame 024799/0055 →
Continuity (3)
Provisional Application 60863721 · Oct 31, 2006
Provisional Application 60942676 · Jun 8, 2007
Related Publication 20080312082A1 · Dec 18, 2008