Thermal reaction device and method for using the same
View Patent ↗An M times.N matrix microfluidic device for performing a matrix of reactions, the device having a plurality of reaction cells in communication with one of either a sample inlet or a reagent inlet through a via formed within an elastomeric block of the device. Methods provided include a method for forming vias in parallel in an elastomeric layer of an elastomeric block of a microfluidic device, the method comprising using patterned photoresist masks and etching reagents to etch away regions or portions of an elastomeric layer of the elastomeric block.
1. A method of analyzing a sample fluid comprising at least one nucleic acid molecule comprising a target sequence, and reagents for amplifying said target sequence, said method comprising the steps of:
i) providing a device having a microfluidic fluid channel therein, said fluid channel having a plurality of valves associated therewith which when activated partition the fluid channel into a plurality of fluidically isolated reaction sites, wherein said fluidically isolated reaction sites are not located at junctions formed by the fluid channel and other channels that are in fluid communication with the fluid channel;
ii) introducing the sample fluid into the fluid channel, wherein the sample fluid contains the target sequence in a concentration such that upon closing the valves to partition the fluid channel into a plurality of fluidically isolated reaction sites, thereby partitioning the sample fluid into a plurality of fluid portions, at least one fluid portion contains the target sequence and at least one fluid portion does not contain the target sequence;
iii) closing the valves to partition the sample fluid into the said fluid portions;
iv) amplifying the target sequence in said fluidically isolated reaction site(s) in which it is present; and
v) determining the number of said fluidically isolated reaction site(s) that contain products of said amplification of said target sequence.
2. A method of claim 1 wherein the amplification is achieved by PCR or by an isothermal amplification reaction.
3. A method of claim 1 wherein the analysis of the sample fluid comprises single nucleotide polymorphism (SNP) analysis.
4. A method of claim 1 wherein the analysis of the sample fluid comprises genotyping analysis.
5. A method of claim 1 wherein the sample fluid comprises at least one nucleic acid molecule comprising a first target sequence and at least one nucleic acid molecule comprising a second target sequence, wherein both the first and second target sequences are amplified, and wherein the products of said amplification of the first and second target sequences are distinguishable.
6. A method of claim 1 wherein determining the number of said fluidically isolated reaction site(s) that contain products of said amplification comprises detecting signal from a dual-labeled fluorogenic oligonucleotide probe in said fluidically isolated reaction site(s).
7. A method of claim 1 wherein the nucleic acid molecule comprising the target sequence is from genomic DNA.
8. A method of claim 7 wherein the nucleic acid molecule is DNA released into a subject's blood stream.