Monoclonal antibodies to human CTLA-8 (IL-17A)
View Patent ↗Human CTLA-8 protein, antibodies that specifically bind to human CTLA-8, and nucleic acids encoding human CTLA-8. Methods of using these molecules and diagnostic kits are also provided.
1. An isolated monoclonal antibody which specifically binds to a human CTLA-8 protein which comprises the amino acid sequence from position 26 to position 155 of SEQ ID NO:8.
2. The monoclonal antibody of claim 1 , which binds to the human CTLA-8 protein with an affinity selected from the group consisting of 100 nM or better, 30 nM or better, 10 nM or better, and 3 nM or better.
3. The monoclonal antibody of claim 2 , which binds to the human CTLA-8 protein with an affinity of 3 nM or better.
4. The monoclonal antibody of claim 1 , which inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.
5. The monoclonal antibody of claim 4 , wherein the human CTLA protein is secreted by activated CD4+ cells.
6. The monoclonal antibody of claim 1 , which is a chimeric antibody or a humanized antibody.
7. The monoclonal antibody of claim 6 , which inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.
8. The monoclonal antibody of claim 1 , which binds to the human CTLA-8 protein with an affinity of 3 nM or better and inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.
9. An antigen binding fragment of an isolated monoclonal antibody which specifically binds to a human CTLA-8 protein wherein said protein comprises the amino acid sequence from position 26 to position 155 of SEQ ID NO:8.
10. The antigen binding fragment of claim 9 , wherein the monoclonal antibody binds to the human CTLA-8 protein with an affinity selected from the group consisting of 100 nM or better, 30 nM or better, 10 nM or better, and 3 nM or better.
11. The antigen binding fragment of claim 10 , wherein the monoclonal antibody binds to the human CTLA-8 protein with an affinity of 3 nM or better.
12. The antigen binding fragment of claim 9 , wherein the monoclonal antibody inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.
13. The antigen binding fragment of claim 12 , wherein the human CTLA-8 protein is secreted by activated CD4+ cells.
14. The binding fragment of claim 9 , wherein the monoclonal antibody is a chimeric antibody or a humanized antibody.
15. The binding fragment of claim 14 , wherein the monoclonal antibody inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.
16. The binding fragment of claim 9 , wherein the monoclonal antibody binds to the human CTLA-8 protein with an affinity of 3 nM or better and inhibits secretion of IL-6 by human synoviocytes incubated with the human CTLA-8 protein.