Plant promoters for use in early seed development
View Patent ↗The present invention relates to DNA molecules that encode transcription regulatory regions. Furthermore, this present invention relates to nucleotide sequences encoding transcription regulatory regions that promote early seed enhanced or seed coat enhanced transcription of contiguous nucleotide sequences.
1. A polynucleotide molecule comprising a sequence selected from the group consisting of:
a) the sequence of SEQ ID NO: 3;
b) a fragment of the sequence of SEQ ID NO: 3 with promoter function; and
c) a polynucleotide sequence comprising at least about 95% identity to the sequence of SEQ ID NO:3, wherein the polynucleotide sequence has promoter function,
wherein said polynucleotide molecule is operably linked to a heterologous transcribable polynucleotide molecule.
2. The polynucleotide molecule of claim 1 , wherein said heterologous transcribable polynucleotide molecule is operably linked to a 3′ transcription termination polynucleotide molecule.
3. The polynucleotide molecule of claim 1 , wherein said heterologous transcribable polynucleotide molecule is a gene of agronomic interest.
4. The polynucleotide molecule of claim 1 , wherein said heterologous transcribable polynucleotide molecule is a marker gene.
5. A transgenic plant stably transformed with the polynucleotide molecule of claim 1 .
6. The transgenic plant of claim 5 , wherein the plant is a transgenic dicotyledonous plant.
7. The transgenic plant of claim 5 , wherein said heterologous transcribable polynucleotide molecule confers altered oil content in the seed to said transgenic plant.
8. The transgenic plant of claim 5 , wherein said heterologous transcribable polynucleotide molecule confers altered protein quality in the seed to said transgenic plant.
9. The transgenic plant of claim 5 , wherein said heterologous transcribable polynucleotide molecule confers altered micronutrient content in the seed to said transgenic plant.
10. A seed of said transgenic plant of claim 5 , wherein the seed comprises said polynucleotide molecule.
11. Meal from said transgenic plant of claim 5 , wherein the meal comprises said polynucleotide molecule.
12. A method of making a vegetable oil and meal, comprising the steps of:
a) incorporating in the genome of a dicotyledonous seed producing, oil-containing plant a polynucleotide molecule according to claim 1 , wherein the transcribable polynucleotide molecule confers altered oil content;
b) growing the dicotyledonous plant to produce seeds; and
c) extracting oil from the seed to produce extracted oil and meal.
13. The polynucleotide molecule of claim 1 , wherein the polynucleotide sequence exhibits at least about 95% identity with the sequence of a).
14. The polynucleotide molecule of claim 1 , wherein the polynucleotide sequence exhibits at least about 98% identity with the sequence of a).
15. The polynucleotide molecule of claim 1 , wherein the polynucleotide sequence exhibits at least about 99% identity with the sequence of a).
16. The polynucleotide molecule of claim 1 , wherein the polynucleotide sequence comprises a fragment of the sequence of SEQ ID NO: 3 with promoter function.
17. The transgenic dicotyledonous plant of claim 6 , wherein said plant is a dicotyledonous plant selected from the group consisting of tobacco, tomato, potato, peanut, soybean, cotton, canola, rapeseed, safflower, flax, sugarbeet, Arabidopsis, Brassica , sunflower, and alfalfa.