Immobilized biologically active entities having a high degree of biological activity
View Patent ↗The present invention relates to immobilized biologically active entities having heparin cofactor II binding activity.
1. A medical device comprising:
a substrate material;
a polymeric covering material attached to at least a portion of a surface of said substrate material, a plurality of biologically active entities having heparin cofactor II binding activity covalently attached to at least a portion of said polymeric covering material; and
a biologically compatible organic composition non-covalently combined with said polymeric covering material and said biologically active entities;
wherein said biologically active entities have a heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ).
2. The medical device of claim 1 , wherein said biologically active entities have a heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ) following sterilization.
3. The medical device of claim 1 , wherein said biologically active entities have a heparin cofactor II binding activity of at least 5 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ) following compaction and expansion.
4. The medical device of claim 1 wherein said plurality of biologically active entities comprises a polysaccharide.
5. The medical device of claim 1 wherein said plurality of biologically active entities comprises a glycosoaminoglycan.
6. The medical device of claim 1 wherein said plurality of biologically active entities comprises dermatan disulfate.
7. The medical device of claim 1 wherein said plurality of biologically active entities comprises end-point attached dermatan disulfate.
8. The medical device of claim 1 wherein said plurality of biologically active entities have an heparin cofactor II binding activity of at least 12 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ).
9. The medical device of claim 1 wherein said plurality of biologically active entities have an heparin cofactor II binding activity of at least 20 picomoles heparin cofactor II per square centimeter (pmol/cm 2 ).