Corn event DAS-59122-7 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. A corn plant comprising in its genome a DNA construct linked to at least one flanking region, wherein:
(a) said flanking region comprises a nucleotide sequence selected from the group consisting of the nucleotide sequence set forth in SEQ ID NO: 19 and the nucleotide sequence set forth in SEQ ID NO: 20;
(b) said DNA construct comprises a first, a second, and a third expression cassette;
(c) said first expression cassette comprises in operable linkage
(i) a maize ubiquitin promoter,
(ii) a 5′ untranslated exon of a maize ubiquitin gene,
(iii) a maize ubiquitin first intron,
(iv) a Cry34Abl encoding DNA molecule, and
(v) a PinII transcriptional terminator;
(d) said second expression cassette comprises in operable linkage
(vi) a wheat peroxidase promoter,
(vii) a Cry35Abl encoding DNA molecule, and
(viii) a PinII transcriptional terminator; and
(e) said third expression cassette comprises in operable linkage
(ix) a cauliflower mosaic virus (CaMV) 35S promoter;
(x) a pat encoding DNA molecule; and
(xi) a 3′ transcriptional terminator from CaMV 35S.
2. The plant of claim 1 , wherein said DNA construct comprises the nucleotide sequence set forth in SEQ ID NO: 24.
3. The plant of claim 1 , wherein said DNA construct is linked to a first and a second flanking region.
4. The plant of claim 3 , wherein said first flanking region comprises the nucleotide sequence set forth in SEQ ID NO: 19 and said second flanking region comprises the nucleotide sequence set forth in SEQ ID NO: 20.
5. The plant of claim 4 , wherein said DNA construct comprises the nucleotide sequence set forth in SEQ ID NO: 24.
6. The plant of claim 1 , wherein said plant is a seed.
7. The plant of claim 2 , wherein said plant is a seed.
8. The plant of claim 4 , wherein said plant is a seed.
9. The plant of claim 5 , wherein said plant is a seed.
10. A plant comprising in its genome the nucleotide sequence set forth in SEQ ID NO: 23.
11. The plant of claim 10 , wherein said plant is a corn plant.
12. A seed comprising in its genome the nucleotide sequence set forth in SEQ ID NO: 23.
13. The seed of claim 12 , wherein said seed is a corn seed.
14. The plant of claim 3 , wherein said plant is a seed.