Corn event DAS-59122-7 and methods for detection thereof
The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
1. A method of detecting the presence of maize event DAS-59122-7 in a biological sample, from a maize plant comprising:
(a) extracting a DNA sample from said biological sample;
(b) providing a pair of DNA primer molecules selected from the group consisting of:
i) the sequences of SEQ ID NO:18 and SEQ ID NO:1;
ii) the sequences of SEQ ID NO:10 and SEQ ID NO:9;
iii) the sequences of SEQ ID NO:2 and SEQ ID NO:17; and
iv) the sequences of SEQ ID NO:8 and SEQ ID NO:17;
(c) providing DNA amplification reaction conditions;
(d) performing said DNA amplification reaction, thereby producing a DNA amplicon molecule; and
(e) detecting said DNA amplicon molecule, wherein the detection of said DNA amplicon molecule in said DNA amplification reaction indicates the presence of maize event DAS-59122-7.