Generation of plants with altered protein, fiber, or oil content
View Patent ↗The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an IMQ nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.
1. A transgenic plant, comprising a plant transformation vector comprising a nucleotide sequence that encodes an IMQ polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 104 or an IMQ polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 104, whereby the transgenic plant has an improved meal quality phenotype, relative to control plants.
2. The transgenic plant of claim 1 , wherein the IMQ polypeptide comprises the amino acid sequence as set forth in SEQ ID NO: 104.
3. The transgenic plant of claim 1 , which is selected from the group consisting of plants of the Brassica species, including canola and rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor, peanut, wheat, oat, and rice.
4. The transgenic plant of claim 3 , wherein the plant is canola.
5. The transgenic plant of claim 1 , wherein an improved meal quality phenotype comprises an increase in available metabolizable energy in meal produced from seeds of the transgenic plant, relative to control plants.
6. The transgenic plant of claim 5 , wherein an increase in available metabolizable energy comprises an altered protein and/or fiber content in the seeds of the transgenic plant.
7. The transgenic plant of claim 6 , wherein the protein content is increased and/or the fiber content is decreased.
8. The transgenic plant of claim 5 , wherein an increase in available metabolizable energy comprises a decreased fiber content in the seeds of the transgenic plant.
9. A plant part obtained from the plant according to claim 1 .
10. The plant part of claim 9 , which is a seed.
11. Meal, feed, or food produced from the seed of claim 10 .
12. A method of producing meal, comprising growing the transgenic plant of claim 1 , and recovering meal from the plant,
thereby producing meal.
13. The method of claim 12 , wherein the meal is produced from seeds of the plant.
14. A feed, meal, grain, food, or seed from the transgenic plant of claim 1 , wherein the feed, meal, grain, food, or seed comprises a nucleic acid sequence encoding a polypeptide comprising an amino acid sequence least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 104, whereby the feed, meal, grain, food, or seed from the transgenic plant has an improved meal quality phenotype, relative to a control plant that does not comprise the nucleic acid.
15. The feed, meal, grain, food, or seed of claim 14 , wherein the polypeptide is encoded by the nucleic acid sequence as set forth in SEQ ID NO: 103.
16. A feed, meal, grain, food, or seed from the transgenic plant of claim 1 , wherein the feed, meal, grain, food, or seed comprises a polypeptide comprising the amino acid sequence as set forth in SEQ ID NO: 104 or a polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 104, whereby the feed, meal, grain, food, or seed from the transgenic plant has an improved meal quality phenotype, relative to a control plants.
17. The feed, meal, grain, food, or seed of claim 16 , wherein the polypeptide comprises an amino acid sequence as set forth in SEQ ID NO: 104.
18. A method of producing an improved meal quality phenotype in a plant, comprising:
a) introducing into progenitor cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes an IMQ polypeptide comprising the amino acid sequence as set forth in SEQ ID NOI: 104 or an IMQ polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence as set forth in SEQ ID NO: 104, and
b) growing the transformed progenitor cells to produce a transgenic plant, wherein the nucleotide sequence is expressed, and the transgenic plant exhibits an improved meal quality phenotype, relative to control plants, thereby producing the improved meal quality phenotype in the plant.
19. The method of claim 18 , wherein the IMQ polypeptide comprises the amino acid sequence as set forth in SEQ ID NO: 104.
20. A plant obtained by a method of claim 18 .
21. The plant of claim 20 , which is selected from the group consisting of plants of the Brassica species, including canola and rapeseed, soy, corn, sunflower, cotton, cocoa, safflower, oil palm, coconut palm, flax, castor, peanut, wheat, oat, and rice.
22. The plant of claim 21 , wherein the plant is canola.
23. The plant of claim 20 , wherein the plant is selected from the group consisting of a plant grown from said progenitor cells, a plant that is the direct progeny of a plant grown from said progenitor cells, and a plant that is the indirect progeny of a plant grown from said progenitor cells.