Nucleic acid encoding PRO10268 polypeptides
View Patent ↗The present invention relates to compositions containing novel proteins and methods of using those compositions for the diagnosis and treatment of immune related diseases.
1. An isolated nucleic acid comprising: (a) a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16); (b) a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16), lacking its associated signal peptide; (c) the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15); (d) the full-length coding sequence of the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15); or (e) the full-length coding sequence of the cDNA deposited under ATCC accession number PTA-1179, wherein the isolated nucleic acid encodes a polypeptide which inhibits the proliferation of T-lymphocytes.
2. The isolated nucleic acid of claim 1 comprising a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16).
3. The isolated nucleic acid of claim 1 comprising a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16), lacking its associated signal peptide.
4. The isolated nucleic acid of claim 1 comprising the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15).
5. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15).
6. The isolated nucleic acid of claim 1 comprising the full-length coding sequence of the cDNA deposited under ATCC accession number PTA-1179.
7. A vector comprising the nucleic acid of claim 1 .
8. The vector of claim 7 , wherein said nucleic acid is operably linked to control sequences.
9. An isolated host cell comprising the vector of claim 7 .
10. The host cell of claim 9 , wherein said cell is a CHO cell, an E. coli or a yeast cell.
11. An isolated nucleic acid that hybridizes under the high stringency conditions of 50% formamide, 5×SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5× Denhardt's solution, sonicated salmon sperm DNA (50 μg/ml), 0.1% SDS, and 10% dextran sulfate at 42° C., with washes at 42° C. in 0.2×SSC (sodium chloride/sodium citrate) and 50° C., formamide at 55° C., followed by a high-stringency wash consisting of 0.1×SSC containing EDTA at 55° C. to:
(a) a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16);
(b) a nucleic acid sequence encoding the polypeptide shown in FIG. 16 (SEQ ID NO: 16), lacking its associated signal peptide;
(c) the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15);
(d) the full-length coding sequence of the nucleic acid sequence shown in FIG. 15 (SEQ ID NO: 15); or
(e) the full-length coding sequence of the cDNA deposited under ATCC accession number PTA-1179.