Skin Model
The present invention relates to the use of a pig skin sample as a skin model, particularly for the assessment of pharmacological and cosmetic effects.
1 - 13 . (canceled)
14 . A method of modeling, simulating or analyzing a selected effect of a selected treatment of human or animal skin, comprising the steps of:
a) providing a skin sample of a pig, wherein the sample comprises an epidermis, dermis and a subcutis layer,
b) subjecting the sample to the selected treatment, and
c) observing the effect of the treatment on the skin sample.
15 . The method of claim 14 , wherein the skin sample comprises at least one primary hair follicle.
16 . The method of claim 15 , wherein the skin sample has a hair follicle density of at least 15 follicles per cm.
17 . The method of claim 14 , wherein the skin sample is a skin sample of a domestic pig.
18 . The method of claim 17 , wherein the domestic pig is a Pietrain or Landrace pig.
19 . The method of claim 14 , wherein the skin is of a dorsal or neck part of the pig.
20 . The method of claim 14 , wherein the skin sample comprises a subcutis layer with an average thickness of at least 1 mm.
21 . The method of claim 14 , wherein the skin sample has a dimension (length×width×thickness) of (3-6)×(3-6)×(2-5) mm.
22 . The method of claim 14 , wherein the effect to be observed is
a) an effect of exposition of the skin sample to substances, the substances being applied topically to the skin sample and/or systemically to the skin sample by mixing with a culture medium, and/or
b) an effect of skin sample irradiation.
23 . The method of claim 14 for the assessment of: modulation of skin and/or hair pigmentation,
modulation of hair growth,
modulation of skin and/or hair viability and/or proliferation,
modulation of fat metabolism,
anti-cellulitis properties of substances,
slimming,
anti-aging effects, particularly by fat cell stimulation allergenic potential and/or irritation,
UV protection, particularly UV erythema prevention, alleviation and
healing,
modulation of connecting tissue properties, particularly for the assessment of anti-wrinkle properties of substances,
anti-oxidative effects,
wound healing,
modulation of skin barrier function,
modulation of ion channels, especially neurofunctional channels and preferably channels activated by GABA, glutamate, acetylcholine, serotonin, adrenalin and ATP, and temperature sensitive channels (TRPMS, TRPV3, TRPV4, TRPV1, ANKTM, TRPV2),
immunestimulation, immunesuppression,
sebum stimulation, sebum suppression,
anti-microbial effectiveness, particularly anti-acne effectiveness,
sweat secretion decrease,
substantivity of materials on a skin surface,
film forming effectiveness,
modulation of hair thickness,
moisturization,
phototoxicity, and/or
skin metabolism of compounds.
24 . A system for modeling a selected effect of a selected treatment of human skin, comprising an isolated skin sample of a pig, the sample including an epidermis, dermis and a subcutis layer.
25 . A system according to claim 24 , wherein the skin sample is a skin sample of a domestic pig.
26 . The system of claim 24 , wherein the pig is a Pietrain or Landrace pig
27 . The system according to claim 24 , wherein the skin sample is placed with its subcutis layer on a support comprising a cultivation medium.