Generation of plants with altered oil, protein, or fiber content
View Patent ↗The present invention is directed to plants that display an improved oil quantity phenotype or an improved meal quality phenotype due to altered expression of an HIO nucleic acid. The invention is further directed to methods of generating plants with an improved oil quantity phenotype or improved meal quality phenotype.
1. A method of producing oil comprising: growing a transgenic plant that comprises a plant transformation vector comprising a nucleotide sequence that encodes a [HIO]polypeptide comprising an amino acid sequence having at least 95% sequence identity to the sequence set forth in SEQ ID NO: 2 and conveying an increased oil phenotype on a plant when expressed in said plant, whereby the transgenic plant has an increased oil content phenotype, relative to a plant of the same species that does not comprise the plant transformation vector; and recovering oil from said transgenic plant.
2. The method of claim 1 , wherein the oil is recovered from a seed of the transgenic plant.
3. A method of producing increased oil content in a plant, said method comprising: introducing into progenitor cells of the plant a plant transformation vector comprising a heterologous constitutive promoter operatively linked to a nucleotide sequence that encodes a [HIO]polypeptide comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 2 and conveying an increased oil phenotype on a plant when expressed in said plant, to produce transformed cells overexpressing the polypeptide;
growing the transformed progenitor cells to produce a transgenic plant, wherein said polynucleotide sequence is expressed; and
identifying said transgenic plant that exhibits the increased oil content phenotype relative to a plant of the same species that does not comprise the plant transformation vector.
4. The method of claim 3 wherein the nucleotide sequence encodes a [HIO]polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 2.
5. The method of claim 3 wherein the nucleotide sequence encodes a [HIO]polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2.
6. The method of claim 3 wherein the nucleotide sequence encodes a [HIO]polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 2.
7. The method of claim 1 wherein the nucleotide sequence encodes a [HIO]polypeptide consisting of an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO: 2.
8. The method of claim 1 wherein the nucleotide sequence encodes a [HIO]polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 2.
9. The method of claim 1 , wherein the nucleotide sequence encodes a [HIO]polypeptide consisting of the amino acid sequence set forth in SEQ ID NO: 2.