Method and system for locating and focusing on fiducial marks on specimen slides
View Patent ↗Methods are disclosed for locating and focusing on a fiducial mark on a specimen slide. A plurality of pixels are identified as candidate pixels. A pixel is identified as a candidate pixel based on a number of empty pixels in an area defined by boundary lines extending from the pixel, and one or more dimensions, such as the perimeter, of the defined area. The candidate pixel enclosing the largest area is selected from the group or set of candidate pixels, and the coordinates of that pixel are considered to be the coordinates of the corner of the fiducial mark. The methods can be performed using different gray values that define dark or fiducial pixels and light or empty pixels. Differences between the results at different gray values can be used as focus scores for automatic focusing on the fiducial mark.
1. A method of locating a corner of a fiducial mark within an image of a specimen slide, the image having a plurality of pixels, the method comprising:
selecting a pixel of the plurality of image pixels, the selected pixel defining an area based on lines extending from the selected pixel, the selected pixel being selected based on a ratio of a number of empty pixels in the defined area and one or more dimensions of the defined area satisfying a threshold; and
determining a location of the corner of the fiducial mark using the selected pixel.
2. The method of claim 1 , wherein the selected pixel defines the largest area compared to other image pixels that satisfy the threshold.
3. The method of claim 1 , wherein the boundary lines are straight.
4. The method of claim 1 , wherein the boundary lines extend from the selected pixel to an edge of the image.
5. The method of claim 1 , wherein an area defined by each pixel is a square or a rectangle.
6. The method of claim 1 , wherein the ratio is a ratio of (number of empty pixels in the area defined by the selected pixel) to (one or more dimensions of the area defined by the selected pixel).
7. A method of locating a corner of a fiducial mark within an image of a specimen slide, the image having a plurality of pixels, the method comprising:
identifying a plurality of pixels as candidate pixels, each candidate pixel being identified based on a number of empty pixels in a bounding area defined by lines extending from the candidate pixel relative to one or more dimensions of the defined area;
selecting one candidate pixel, wherein lines extending from the selected pixel define the largest bounding area compared to lines extending from other candidate pixels; and
determining a location of the corner of the fiducial mark based on the selected candidate pixel.
8. The method of claim 7 , wherein the bounding area corresponding to the selected pixel contains the largest number of dark pixels compared to bounding areas defined by other candidate pixels.
9. The method of claim 7 , wherein the lines are straight.
10. The method of claim 7 , wherein the lines extend from the selected candidate pixel to an edge of the image.
11. The method of claim 7 , wherein the bounding area defined by each candidate pixel is a box or a rectangle.
12. The method of claim 7 , wherein each candidate pixel is identified based on a ratio satisfying a threshold, the ratio comprising a ratio of a number of empty pixels in the bounding area defined by lines extending from the selected candidate pixel to one or more dimensions of the bounding area.
13. The method of claim 12 , wherein each candidate pixel is selected based on a ratio of the number of empty pixels in the bounding area to one or more dimensions of the bounding area being below a threshold.
14. The method of claim 13 , wherein the ratio is a ratio of the number of empty pixels in the bounding area to the perimeter of the bounding area.
15. The method of claim 14 , wherein the ratio is a ratio of the number of empty pixels in the bounding area to the semiperimeter of the bounding area.
16. The method of claim 7 performed in the presence of an air bubble or debris overlapping the fiducial mark.
17. The method of claim 7 , wherein a pixel is treated as an empty pixel if a gray value of the pixel is greater than 128.
18. The method of claim 7 performed at multiple gray value thresholds.
19. The method of claim 18 , further comprising
calculating a focus score based on a difference between a first bounding area and a second bounding area.
20. The method of claim 19 , wherein the difference is the Euclidean distance between corners of the first and second bounding areas.
21. The method of claim 7 performed at multiple gray value thresholds, wherein
a first bounding area is identified using a first empty pixel threshold to identify a first location of the corner of the fiducial mark, and
a second bounding area is identified using a second empty pixel threshold to identify a second location of the corner of the fiducial mark.
22. The method of claim 21 , further comprising
calculating a focus score based on a distance between the first and second bounding areas.
23. The method of claim 22 , further comprising automatically focusing an image device based on the calculated focus score.
24. The method of claim 22 , wherein the distance is the Euclidean distance between the corners of the first and second bounding areas.