Provided is an assay device and kit for detecting the presence or amount of an analyte of interest.
1. An assay device for determining the presence and/or amount of an analyte of interest in a sample, the assay device comprising:
a sampling device, the sampling device comprising at least one puncturing element for opening a puncturable liquid seal;
a housing for receiving the sampling device, the housing comprising a neck portion, a liquid container portion, and an assay means;
wherein the liquid container comprises a first puncturable liquid seal and a second puncturable liquid seal, wherein the second puncturable liquid seal fluidically separates the liquid container from the assay means; and
wherein the sample contacts the assay device when the second puncturable liquid seal is punctured by the sampling device.
2. The assay device according to claim 1 , wherein the liquid container comprises a single liquid chamber.
3. The assay device according to claim 1 , wherein the liquid pathway comprises a porous flow through matrix, wherein the porous flow through matrix comprises a lateral flow carrier.
4. The assay device according to claim 3 , wherein the lateral flow carrier comprises a plurality of porous materials.
5. The assay device according to claim 1 , wherein the neck portion comprises one or more projections extending into the bore of the neck portion.
6. The assay device according to claim 5 , wherein the one or more projections are one or more fins.