DAP12 nucleic acids
View Patent ↗The purification and isolation of various genes which encode mammalian cell surface polypeptides. Nucleic acids, proteins, antibodies, and other reagents useful in modulating development of cells, e.g., lymphoid and myeloid, are provided, along with methods for their use.
1. An isolated or purified nucleic acid encoding a polypeptide comprising the sequence of SEQ ID NO 2.
2. An expression vector comprising the nucleic acid of claim 1 .
3. A host cell comprising the expression vector of claim 2 .
4. The host cell of claim 3 , wherein the host cell is:
a) a prokaryotic cell;
b) a eukaryotic cell;
c) an insect cell; or
d) a yeast cell.
5. A method of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:2 comprising:
a) culturing the host cell of claim 4 under conditions suitable for expression of the polypeptide; and
b) isolating the polypeptide.
6. The nucleic acid of claim 1 , wherein the nucleic acid comprises SEQ ID NO:1.
7. The nucleic acid of claim 6 , wherein the nucleic acid is detectably labeled.
8. A method of making a nucleic acid duplex comprising contacting the nucleic acid of claim 1 with a complementary nucleic acid under conditions suitable for the formation of the duplex and detecting the duplex.