Novel receptors
The invention provides isolated nucleic acid and amino acid sequences of novel receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of the receptors.
1 . An antibody that selectively binds to a TGR343 protein which comprises the amino acid sequence of SEQ ID NO:10.
2 . The antibody of claim 1 which is selected from the group consisting of a fragment of an antibody, a monoclonal antibody, and a polyclonal antibody.
3 . The antibody of claim 2 which is the fragment and wherein the fragment is selected from the group consisting of a Fab fragment, a Fab′ fragment, and a F(ab′) 2 fragment.
4 . The antibody of claim 1 further comprising a detectable label.
5 . The antibody of claim 4 wherein the detectable label is selected from the group consisting of a fluorescent dye, a radiolabel, an enzyme, and a calorimetric label.
6 . The antibody of claim 1 which is recombinantly produced.
7 . The antibody of claim 1 which is immobilized on a solid support.
8 . The antibody of claim 1 which further comprises biotin.
9 . A method of detecting TGR343 in a sample comprising:
(i) isolating a biological sample;
(ii) contacting the biological sample with an antibody which selectively binds to a TGR343 protein which comprises the amino acid sequence of SEQ ID NO:10; and
(iii) detecting selective association of the antibody with the biological sample.
10 . The method of claim 9 wherein the detection is by immunoassay.
11 . The method of claim 9 wherein the biological sample is a tissue.
12 . The method of claim 9 wherein the biological sample is from a patient suspected to have Multiple Sclerosis.
13 . The method of claim 9 wherein the antibody is bound to a solid support.
14 . The method of claim 9 wherein the antibody is selected from the group consisting of a monoclonal antibody, a polyclonal antibody, an Fab fragment, an Fab′ fragment, or an F(ab′) 2 fragment.
15 . A purified molecule selected from the group consisting of:
a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12;
a nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11; and
a polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12.
16 . The purified molecule of claim 15 which is the polypeptide.
17 . The purified molecule of claim 15 which is the nucleic acid molecule encoding the polypeptide which comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12.
18 . The purified molecule of claim 15 which is the nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:9, and SEQ ID NO:11.