Protein chip and production method thereof
View Patent ↗A protein chip including at least a substrate having a plurality of steps which are regularly arranged on one surface thereof; a plurality of metallic microstructures arranged in the steps; and a lipid vesicle in which an outer surface thereof is modified by a functional group and a protein is present in a lipid bilayer thereof. The metallic microstructures and the lipid vesicle are bound via the functional group to provide the protein on the substrate.
1. A protein chip comprising:
a substrate having a plurality of steps which are regularly arranged on one surface thereof;
a plurality of metallic microstructures arranged in the steps; and
a lipid vesicle bound to each of the plurality of metallic microstructures, each lipid vesicle having a lipid bilayer and a functional group arranged on an outer surface thereof, where a membrane protein selected from the group consisting of an ion channel receptor, a G-protein coupled receptor, and an ion channel is present in the lipid bilayer,
wherein the metallic microstructures and the lipid vesicles are bound via the functional group to provide the protein on the substrate,
wherein a density of the metallic microstructures is 10 12 to 10 14 per m 2 , and
wherein the lipid vesicles are arranged on the substrate at a density of 10 12 to 10 14 vesicles per m 2 .
2. The protein chip according to claim 1 , wherein the metallic microstructures are comprised of gold.
3. The protein chip according to claim 1 , wherein the metallic microstructures form a nanodot array.
4. The protein chip according to claim 1 , wherein the functional group is any one selected from the group consisting of a thiol group, an amide group, a sulfide group, a thioacetyl group, and a disulfide group.
5. The protein chip according to claim 1 , wherein the membrane protein is a TRP channel, an ATP receptor, a serotonin receptor, an NMDA receptor, an AMPA receptor, a kainate receptor, a GABA receptor, an adenosine receptor, an adrenaline receptor, a metabotropic glutamate receptor, or an opioid receptor.
6. A method for manufacturing the protein chip according to any one of claims 1 to 4 and 5 , comprising:
arranging a plurality of metallic microstructures onto a plurality of steps which are arranged regularly on one surface of a substrate; and
arranging a protein on the substrate by binding a lipid vesicle, in which an outer surface thereof is modified by a functional group and a protein is present in a lipid bilayer thereof, to the metallic microstructures via the functional group.