Functional and hyperfunctional siRNA directed against Bcl-2
Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rationale design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing Bcl-2.
1 . An siRNA molecule effective at silencing Bcl-2 expression, wherein said: molecule comprises a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region comprising 19-30 base pairs and said antisense region comprises a sequence that is the reverse complement of SEQ. ID NO. 302.
2 . The siRNA of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3 . The siRNA of claim 1 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4 . The siRNA of claim 1 , wherein said antisense region is 100% complementary to a target Bcl-2 RNA.
5 . The siRNA molecule of claim 1 , wherein said siRNA molecule comprises at least one overhang region, wherein said overhang region comprises six or fewer nucleotides.
6 . The siRNA molecule of claim 1 wherein said siRNA molecule comprises no overhang regions.
7 . The siRNA molecule of claim 2 wherein said siRNA molecule comprises at least one overhang region, wherein said overhang region comprises six or fewer nucleotides.
8 . The siRNA molecule of claim 2 wherein said siRNA molecule comprises no overhang regions.
9 . The siRNA molecule of claim 3 wherein said siRNA molecule comprises at least one overhang region, wherein said overhang region comprises six or fewer nucleotides.
10 . The siRNA molecule of claim 3 wherein said siRNA molecule comprises no overhang regions.