IP Library › Granted Patent US 7,521,214
Granted Patent B2
US 7,521,214 · App. 11/976,951 · Granted Apr 21, 2009

Method of immobilizing malate dehydrogenase on a substrate

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Quick Facts
Patent No.
US 7,521,214
App. No.
11/976,951
Granted
Apr 21, 2009
Kind
B2
Abstract

Immobilization of malate dehydrogenase on a substrate using a glycerol solution containing malate dehydrogenase is achieved through dropping a mixed solution obtained by adding at least one selected from malic acid and malate to the glycerol containing malate dehydrogenase on the substrate, and drying it thereon. It is preferable to prepare the mixed solution by adding the malate to the glycerol solution containing malate dehydrogenase. The malate is preferably at least one selected from potassium malate and sodium malate.

Claims (5)

1. A method of immobilizing malate dehydrogenase on a substrate, the method comprising:

placing a mixed solution on a substrate, the mixed solution being obtained by adding at least one selected from malic acid and malate to a glycerol that contains malate dehydrogenase; and

drying the mixed solution to immobilize the malate dehydrogenase on the substrate.

2. The method according to claim 1 , wherein the malate includes at least one selected from potassium malate and sodium malate.

3. The method according to claim 1 , wherein the mixed solution is obtained by adding the malate to the glycerol.

Assignments (2)
CHANGE OF NAME Recorded Nov 19, 2008
From: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
To: PANASONIC CORPORATION
Reel/Frame 021858/0958 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 11, 2008
From: YAKU, HIDENOBU; SUGIHARA, HIROKAZU
To: MATSUSHITA ELECTRIC INDUSTRIAL CO., LTD.
Reel/Frame 020791/0267 →