siRNA targeting transient receptor potential cation channel, subfamily V, member 1 (TRPV1)
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for TRPV1.
1. An siRNA molecule consisting of: (a) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides; and (b) a sense region and an antisense region, wherein said sense region and said antisense region together form a duplex region that is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO:547.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4. The siRNA molecule of claim 1 , wherein said siRNA molecule has at least one overhang region.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule has no overhang regions.
6. The siRNA molecule of claim 2 , wherein said siRNA molecule has at least one overhang region.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule has no overhang regions.
8. The siRNA molecule of claim 3 , wherein said siRNA molecule has at least one overhang region.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule has no overhang regions.
10. A chemically synthesized double stranded siRNA molecule, wherein:
(a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and
(b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 547.
11. The chemically synthesized double stranded siRNA molecule of claim 10 , wherein each strand of said siRNA molecule is 19-25 nucleotides in length.
12. The chemically synthesized double stranded siRNA molecule of claim 10 , wherein each strand of said siRNA molecule is 19 nucleotides in length.
13. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein said first siRNA is the siRNA molecule of claim 10 , and said second siRNA consists of: (a) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides; and (b) a sense region and an antisense region that together form a duplex region of 19-30 base pairs and said antisense region of said second siRNA molecule has a sequence that is the complement of SEQ. ID. NO. 490.
14. The pool of claim 13 , wherein said first siRNA and said second siRNA each comprise no overhang regions.
15. The pool of claim 13 , wherein said duplex region of said first siRNA and said duplex region of second siRNA are each 19 base pairs in length.
16. The pool of claim 13 , wherein said duplex region of said first siRNA and said duplex region of said second siRNA are each 19-25 base pairs in length.
17. The pool of claim 13 , wherein said first siRNA and said second siRNA each have at least one overhang region.
18. An siRNA molecule consisting of:
(a) a duplex region; and
(b) either no overhang regions or at least one overhang region, wherein each overhang region has six or fewer nucleotides,
wherein the duplex region is nineteen base pairs in length and consists of a sense region that has a sequence that is the same as SEQ ID NO: 547 and an antisense region that has a sequence that is the complement of SEQ ID NO 547.
19. The siRNA molecule of claim 18 , wherein said siRNA molecule has no overhang regions.
20. The siRNA molecule of claim 18 , wherein said siRNA molecule has at least one overhang region.