Detection of analytes using reorganization energy
The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.
1. A method of detecting a target analyte in a test sample, said method comprising:
(1) adding a target analyte to a composition comprising an electrode, said electrode comprising:
(a) a redox active complex comprising:
(i) a redox active molecule, wherein said redox active molecule is a transition metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold; and
(ii) a binding ligand, wherein at least one atom of the binding ligand serves as a coordination moiety to said transition metal to form said redox active complex, and wherein said redox active complex is attached to said electrode via a spacer; and
(b) a self-assembled monolayer; and
(2) detecting said target analyte.
2. The method according to claim 1 , wherein said metal is osmium.
3. The method according to claim 1 , wherein said attachment of said redox active molecule and said binding ligand have a conformation of:
wherein the hashed mark represents said electrode, M is said metal ion, L is a co-ligand, n is independently either 0 or 1 with the proviso that there is at least one L, X is a spacer, BL is said binding ligand, F 1 is a linkage that allows the covalent attachment of said electrode and said spacer, and F 2 is a linkage that allows the covalent attachment of said spacer to said redox active molecule.
4. The method according to claim 1 , wherein said redox active molecule comprises a cyano group.
5. The method according to claim 1 , wherein said binding ligand is a protein.
6. The method according to claim 5 , wherein said protein is a peptide.
7. The method according to claim 1 , wherein said target analyte is an enzyme and said binding ligand is a substrate of said enzyme.
8. The method according to claim 1 , wherein said spacer is an insulator.
9. The method according to claim 1 , wherein said spacer is conductive oligomer.
10. The method according to claim 1 , wherein said detecting step is carried out using voltammetry.
11. A method of detecting a target analyte in a test sample, said method comprising:
(1) adding a target analyte to the composition comprising an electrode, said electrode comprising:
(a) a redox active complex comprising:
(i) a redox active molecule, said redox active molecule comprising osmium and at least one cyano group; and
(ii) a binding ligand comprising a peptide, wherein at least one atom of the binding ligand serves as a coordination moiety to said osmium to form said redox active complex, and wherein said redox active complex is attached to said electrode via a spacer; and
(b) a self-assembled monolayer; and
(2) detecting said target analyte.