siRNA targeting myeloid differentiation primary response gene (88) (MYD88)
View Patent ↗Efficient sequence specific gene silencing is possible through the use of siRNA technology. By selecting particular siRNAs by rational design, one can maximize the generation of an effective gene silencing reagent, as well as methods for silencing genes. Methods, compositions, and kits generated through rational design of siRNAs are disclosed including those directed to nucleotide sequences for MYD88.
1. An siRNA molecule, wherein said siRNA molecule consists of a duplex region and either no overhang regions or at least one overhang region that contains six or fewer nucleotides, wherein the duplex region comprises a sense region and an antisense region, wherein said sense region and said antisense region together form said duplex region and said duplex region is 19-30 base pairs in length and said antisense region comprises a sequence that is the complement of SEQ ID NO: 528 or SEQ ID NO: 476.
2. The siRNA molecule of claim 1 , wherein said antisense region and said sense region are each 19-25 nucleotides in length.
3. The siRNA molecule of claim 2 , wherein said antisense region and said sense region are each 19 nucleotides in length.
4. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises at least one overhang region.
5. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises no overhang regions.
6. The siRNA molecule of claim 2 , wherein said siRNA molecule comprises at least one overhang region.
7. The siRNA molecule of claim 2 , wherein said siRNA molecule comprises no overhang regions.
8. The siRNA molecule of claim 3 , wherein said siRNA molecule comprises at least one overhang region.
9. The siRNA molecule of claim 3 , wherein said siRNA molecule comprises no overhang regions.
10. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 528.
11. The siRNA molecule of claim 1 , wherein said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 476.
12. A chemically synthesized siRNA molecule, wherein:
(a) each strand of said siRNA molecule is between 19 and 30 nucleotides in length; and
(b) one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 528 or SEQ ID NO: 476.
13. The chemically synthesized siRNA molecule of claim 12 , wherein each strand of said siRNA molecule is 19-25 nucleotides in length.
14. The chemically synthesized siRNA molecule of claim 12 , wherein each strand of said siRNA molecule is 19 nucleotides in length.
15. The chemically synthesized siRNA molecule of claim 12 , wherein said one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 528.
16. The chemically synthesized siRNA molecule of claim 12 , wherein said one strand of said siRNA molecule comprises a sequence that is the complement of SEQ ID NO: 476.
17. A pool of at least two siRNAs, wherein said pool comprises a first siRNA and a second siRNA, wherein said first siRNA has a first duplex region and either no overhang regions or at least one overhang region that contains six or fewer nucleotides, wherein the first duplex region comprises a first sense region and a first antisense region, wherein said first sense region and said first antisense region together form said first duplex region and said first duplex region is 19-30 base pairs in length and said first antisense region comprises a sequence that is the complement of SEQ ID NO: 528 and said second siRNA has a second duplex region and either no overhang regions or at least one overhang region that contains six or fewer nucleotides, wherein the second duplex region of said second siRNA comprises a second sense region and a second antisense region, wherein said second sense region and said second antisense region together form said second duplex region and said second duplex region is 19-30 base pairs in length and said second antisense region comprises a sequence that is the complement of SEQ ID NO: 476.
18. The pool of claim 17 , wherein said first siRNA and said second siRNA each comprise no overhang regions.
19. The pool of claim 17 , wherein said duplex region of said first siRNA and said duplex region of second siRNA are each 19-25 base pairs in length.
20. The pool of claim 19 , wherein said duplex region of said first siRNA and said duplex region of second siRNA are each 19 base pairs in length.