Use of microfluidic systems in the electrochemical detection of target analytes

1. A microfluidic device for the detection of a target analyte in a fluid sample, said device comprising a solid support member comprising:

a) a sample inlet port;

b) a configuration of electrodes for an electrokinetic movement of said sample on said solid support member upon application of a voltage;

c) a detection chamber comprising a plurality of detection electrodes positioned in said detection chamber, each comprising a binding ligand and a self assembled monolayer, wherein said detection chamber is in fluid connection to said sample inlet port;

d) at least one storage module comprising at least one dried reagent in fluid connection with said detection chamber,

wherein said electrokinetic movement is electroosmotic movement or electrohydrodynamic movement.

2. The microfluidic device according to claim 1 wherein said electrokinetic movement is electroosmotic movement.

3. The microfluidic device according to claim 1 wherein said electrokinetic movement is electrohydrodynamic movement.

4. The microfluidic device according to claim 1 wherein said binding ligand is a nucleic acid.

5. The microfluidic device according to claim 1 wherein said binding ligand is a protein.

6. The microfluidic device according to claim 1 further comprising a second storage module containing one or more assay reagents.

7. The microfluidic device according to claim 6 wherein at least one of said storage modules contains an enzyme.

8. The microfluidic device according to claim 7 wherein said enzyme is a polymerase.

9. A method for the detection of a target analyte in a sample, said method comprising:

a) introducing said sample to a sample inlet port of a microfluidic device according to claim 1 ;

b) applying a voltage to said configuration of electrodes to move said sample to said detection chamber;

c) forming an attachment complex between said target analyte and at least one of said binding ligands, wherein said attachment complex comprises an electron transfer moiety label; and

d) detecting the presence of said label as an indication of the presence of said target.

10. A method according to claim 9 wherein said electron transfer moiety is a metallocene.

11. A method according to claim 10 wherein said metallocene is a ferrocene.

12. A method according to claim 9 wherein said attachment complex comprises said target analyte, said capture binding ligand, and a solution binding ligand comprising said electron transfer moiety label.

13. A microfluidic device for the detection of a target analyte in a fluid sample, said device comprising a solid support member comprising:

a) a sample inlet port;

b) a configuration of electrodes for an electrokinetic movement of said sample on said solid support member upon application of a voltage;

c) a detection chamber comprising a plurality of detection electrodes positioned in said detection chamber, each comprising a binding ligand and a self-assembled monolayer, wherein said detection chamber is in fluid connection to said sample inlet port;

d) at least one storage module comprising at least one dried reagent in fluid connection with said detection chamber,

wherein said binding ligand is a nucleic acid or a protein.

14. The microfluidic device of claim 13 , wherein said electrokinetic movement is electroosmotic movement.

15. The microfluidic device of claim 13 , wherein said electrokinetic movement is electrohydrodynamic movement.

16. The microfluidic device of claim 13 , wherein said binding ligand is a nucleic acid.

17. The microfluidic device of claim 13 , wherein said binding ligand is a protein.

18. The microfluidic device of claim 13 , further comprising a second storage module containing one or more assay reagents.

19. The microfluidic device of claim 18 , wherein at least one of said storage modules contains an enzyme.

20. The microfluidic device of claim 19 , wherein said enzyme is a polymerase.

21. A method for the detection of a target analyte in a sample, said method comprising:

a) introducing said sample to a sample inlet port of a microfluidic device according to claim 13 ;

b) applying a voltage to said configuration of electrodes to move said sample to said detection chamber;

c) forming an attachment complex between said target analyte and at least one of said binding ligands, wherein said attachment complex comprises an electron transfer moiety label; and

d) detecting the presence of said label as an indication of the presence of said target.

22. The method of claim 21 , wherein said electron transfer moiety is a metallocene.

23. The method of claim 22 , wherein said metallocene is a ferrocene.

24. The method of claim 21 , wherein said attachment complex comprises said target analyte, said capture binding ligand, and a solution binding ligand comprising said electron transfer moiety label.