Detection of target analytes using particles and electrodes
The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.
1 . A composition comprising a colloid particle comprising at least one electron transfer moiety (ETM).
2 . A composition according to claim 1 wherein said particle further comprising a self-assembled monolayer (SAM).
3 . A composition according to claim 1 wherein said colloid further comprises at least one covalently attached nucleic acid.
4 . A composition comprising a colloid particle comprising a covalently attached conductive oligomer.
5 . A composition according to claim 4 wherein said colloid particle further comprises at one ETM.
6 . A composition comprising a colloid particle comprising:
a) a SAM;
b) a capture probe;
c) an amplification sequence; and
d) a label probe hybridized to said amplification sequence, wherein said label probe comprises at least one covalently attached ETM.