Photonic crystal sensors with intergrated fluid containment structure
View Patent ↗Photonic crystal (PC) sensors, and sensor arrays and sensing systems incorporating PC sensors are described which have integrated fluid containment and/or fluid handling structures. Sensors and sensing systems of the present disclosure are capable of high throughput sensing of analytes in fluid samples, bulk refractive index detection, and label-free detection of a range of molecules, including biomolecules and therapeutic candidates. The present disclosure also provides a commercially attractive fabrication platform for making photonic crystal sensors and systems wherein an integrated fluid containment structure and a photonic crystal structure are fabricated in a single molding or imprinting processing step amendable to high throughput processing.
1. A photonic crystal biosensor with an integrated fluid containment structure, the biosensor adapted for measurement of a sample, comprising:
a fluid containment structure having a cavity for receiving said sample, said cavity having at least one internal surface;
and a photonic crystal structure comprising a periodic surface grating structure formed in the internal surface of said fluid containment structure;
wherein said fluid containment structure and periodic surface grating structure of said photonic crystal sensor comprise an integral, monolithic structure.
2. The biosensor of claim 1 , wherein said integral, monolithic structure comprises an integral polymer structure comprising an optically clear substrate layer, a cured polymer layer, and a relatively high index of refraction material deposited on the cured polymer layer.
3. The biosensor of claim 1 , wherein said cavity comprises a sample well, and wherein the integral monolithic structure further comprises a port for receiving the sample, a channel having a surface, the channel providing a fluid path for connecting the port to the fluid containment structure, and a second photonic crystal structure comprising a periodic surface grating structure formed in a surface of the channel.
4. The biosensor of claim 1 wherein said periodic surface grating structure comprises a nanosized structure, and wherein said cavity of said fluid containment structure comprises a micron-sized feature.
5. The biosensor of claim 4 wherein said periodic surface grating structure is formed on a bottom surface of said cavity and wherein said periodic surface grating structure extends from a first side of said cavity to a second side of said cavity.
6. The biosensor of claim 1 , wherein the fluid containment structure comprises a microfluidic flow channel, with the sample flowing through the flow channel over the photonic crystal structure.
7. The biosensor of claim 1 , wherein the fluid containment structure comprises a microwell.
8. The biosensor of claim 1 further comprising a cover layer enclosing said fluid containment structure.
9. The biosensor of claim 8 , wherein said cover layer has an inlet hole and an outlet hole for conducting the sample into and out of the fluid containment structure.
10. The biosensor of claim 1 wherein the integral, monolithic structure further comprises a polymer substrate supporting said fluid containment structure and said photonic crystal structure.
11. The biosensor of claim 1 further comprising a target material bound to the periodic surface grating structure of the photonic crystal structure.
12. The biosensor of claim 11 , wherein said target material is selected from the group of target materials consisting of proteins, peptides, DNA molecules, RNA molecules, oligonucleotides, lipids, carbohydrates, polysaccharides; glycoproteins, lipoproteins, sugars, cells, bacteria, virus, and candidate molecules.
13. A biosensor comprising: an integral, monolithic structure having a port, a plurality of sample wells connected to the port, and a plurality of flow channels connecting the port to the sample wells, and a plurality of photonic crystal sensors integrally formed in the structure, wherein the sample wells and photonic crystal sensors are comprised of a structurally continuous material, each of the sensors positioned in a flow channel connecting the port to the sample wells.
14. The biosensor of claim 13 , wherein each of the flow channels have substantially the same path length between the inlet port and the sample wells.
15. The biosensor of claim 13 , wherein the plurality of photonic crystal sensors are spatially arranged in an aligned condition.
16. The biosensor of claim 13 , wherein the biosensor comprises an array of N×M sample wells, where N represents an integer number of rows of sample wells and M represents an integer number of columns of wells, wherein the biosensor includes an inlet port for each of the M columns of wells, and wherein each of the inlet ports are connected to each of the N wells in each column of wells by a flow channel, each flow channel in the biosensor incorporating a photonic crystal sensor.
17. The biosensor of claim 16 , wherein each of the columns of wells are in a substantially aligned condition and wherein the flow channels are constructed such that the photonic crystal sensors in the flow channels for each column of wells are also in a substantially aligned condition.
18. The biosensor of claim 13 , wherein each of the sample wells includes a photonic crystal sensor positioned within each of the sample wells.